BACKGROUND Multiple sclerosis (MS) is known as one of the chronic inflammatory diseases characterized by lesions in the central nervous system (CNS) and peripheral nervous system(PNS) resulting in serious cognitive or physical disabilities as well as neurological disorders. Thus, protective effects of erythropoietin(EPO) on myelinization of oligodendrocytes and schwann cells respectively in CNS and PNS following MS induced by cuprizone (CPZ) administration in young female mice. METHODOLOGY To meet the objectives of this study; a chow with 0.2% CPZ was used to feed young female C57BL/6 J mice for six weeks. After three weeks, EPO (5,000 IU/kg body weight) was administered via daily intra-peritoneal injection for simultaneous treatment of the mice. Measurement of latency and amplitude of the compound muscle action potential (CMAP) of gastrocnemius muscle was also performed every week during a six-week demyelination interval, and then examinations were fulfilled on the histological sections of the brain and sciatic nerve. Therefore, we focused on the removal of the sciatic and sciatic nerve specimens and analysis of the use of the stereological procedures, western blot, immuno-histochemistry, and gene expression. RESULTS According to the results of this study, MBP levels increased in oligodendrocytes (OLs) in the treated mice. Moreover, EPO could concurrently enhance motor coordination and muscle activity. Analysis showed the significant enhancement of the gene expression of MBP, MAG, and S100, as well as stereological variables in the treatment group in comparison with the cuprizone (CPZ) group. CONCLUSION Findings could help further understand the alleviation of the detrimental impacts of CPZ using the OLs that would be capable of increasing the level of S100, MAG, and MBP.

中文翻译：

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促红细胞生成素对铜宗诱导的多发性硬化症后 CNS 和 PNS 中少突胶质细胞和雪旺细胞髓鞘化的保护作用 - 组织学、分子和功能研究


背景技术多发性硬化症(MS)被认为是一种慢性炎症性疾病，其特征在于中枢神经系统(CNS)和周围神经系统(PNS)的病变，导致严重的认知或身体残疾以及神经障碍。因此，在年轻雌性小鼠中，在给予铜宗（CPZ）诱导MS后，促红细胞生成素（EPO）对CNS和PNS中少突胶质细胞和雪旺细胞的髓鞘化有保护作用。方法论 实现本研究的目标；使用含有 0.2% CPZ 的饲料喂养年轻雌性 C57BL/6 J 小鼠六周。三周后，每日腹腔注射EPO（5,000 IU/kg体重），同时对小鼠进行治疗。在六周的脱髓鞘间隔期间，每周测量腓肠肌复合肌肉动作电位（CMAP）的潜伏期和振幅，然后对大脑和坐骨神经进行组织学切片检查。因此，我们重点关注坐骨和坐骨神经标本的取出，并使用体视学程序、蛋白质印迹、免疫组织化学和基因表达进行分析。结果 根据这项研究的结果，接受治疗的小鼠少突胶质细胞 (OL) 中的 MBP 水平升高。此外，EPO可以同时增强运动协调性和肌肉活动。分析显示，与铜宗 (CPZ) 组相比，治疗组的 MBP、MAG 和 S100 基因表达以及体视学变量显着增强。 结论 研究结果有助于进一步了解使用能够提高 S100、MAG 和 MBP 水平的 OL 来减轻 CPZ 的有害影响。