Immunostaining for calbindin (CB) is commonly used to label particular populations of neurons. Recently, it has been shown that the CA1 pyramidal cells in the mouse can be subdivided along the radial axis into superficial and deep pyramidal cells and that this segregation in the radial axis may represent a general principle of structural and functional organization of the hippocampus. One of the most widely used markers of the superficial pyramidal cells is CB. However, this laminar segregation of pyramidal cells has not been reported in the human CA1 using CB immunostaining. The problem is that the different pattern of CB immunostaining observed in the mouse compared to the human could be explained by technical features, of which one of the most important is the postmortem time (PT) delay typical of the brain tissue obtained from humans. In the present study, we have studied the influences of PT delays and fixation procedures and we found that the clear differences found between the CA1 of the human and mouse do not depend on the fixation, but represent actual species-specific differences. These remarkable differences between species should be taken into consideration when making interpretations in translational studies from mouse to human brains.

中文翻译：

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人和小鼠 CA1 海马锥体细胞层钙结合蛋白免疫染色：比较研究


钙结合蛋白 (CB) 免疫染色通常用于标记特定的神经元群体。最近，研究表明，小鼠的 CA1 锥体细胞可以沿径向轴细分为浅层和深层锥体细胞，这种径向轴的分离可能代表了海马结构和功能组织的一般原则。浅表锥体细胞最广泛使用的标记物之一是 CB。然而，使用 CB 免疫染色在人类 CA1 中尚未报道锥体细胞的这种层状分离。问题在于，与人类相比，在小鼠中观察到的 CB 免疫染色模式不同可以用技术特征来解释，其中最重要的特征之一是从人类获得的脑组织典型的死后时间 (PT) 延迟。在本研究中，我们研究了 PT 延迟和固定程序的影响，我们发现人类和小鼠 CA1 之间的明显差异并不取决于固定，而是代表了实际的物种特异性差异。在对从小鼠大脑到人类大脑的转化研究进行解释时，应该考虑到物种之间的这些显着差异。