MicroRNAs (miRNAs) regulate genes through post-transcriptional regulation by targeting the 3'-UTR of mRNA to downregulate gene expression. Several reports have indicated that miRNA regulation can affect many physiological processes, including immune function, apoptosis, cell proliferation and differentiation, and milk fat metabolism. In this study, miR-142-5P promoted milk fat metabolism, and inhibition of miR-142-5P suppressed milk fat metabolism both in vivo and in vitro. The luciferase and Western blot assays indicated that catenin beta-1 (CTNNB1) is a potential target for miR-142-5P. In addition, CTNNB1 inhibited milk fat metabolism. In summary, miR-142-5P may promote milk fat metabolism by inhibiting CTNNB1 expression.

中文翻译：

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miR-142-5P通过靶向CTNNB1调节山羊乳腺上皮细胞中的甘油三酸酯。

MicroRNA（miRNA）通过转录后调控，通过靶向mRNA的3'-UTR来下调基因表达，从而调控基因。几篇报道表明，miRNA调节可影响许多生理过程，包括免疫功能，细胞凋亡，细胞增殖和分化以及乳脂代谢。在这项研究中，miR-142-5P促进了乳脂的代谢，而抑制miR-142-5P则在体内和体外均抑制了乳脂的代谢。荧光素酶和蛋白质印迹试验表明，连环蛋白β-1（CTNNB1）是miR-142-5P的潜在靶标。此外，CTNNB1抑制乳脂代谢。总之，miR-142-5P可通过抑制CTNNB1表达来促进乳脂代谢。