BACKGROUND:Obesity-induced chronic inflammation and fibrosis in adipose tissue contributes to the progression of type 2 diabetes mellitus (DM). While fibrosis is known to induce mechanical stiffening of numerous tissue types, it is unknown whether DM is associated with alterations in adipose tissuemechanical properties. OBJECTIVE:The purpose of this study was to investigate whether DM is associated with differences in bulk viscoelastic properties of adipose tissue from diabetic (DM) and non-diabetic (NDM) obese subjects. METHODS:Bulk shear rheology was performed on visceral (VAT) and subcutaneous (SAT) adipose tissue, collected from obese subjects undergoing elective bariatric surgery. Rheology was also performed on the remaining extracellular matrix (ECM) from decellularized VAT (VAT ECM). Linear mixed models were used to assess whether correlations existed between adipose tissue mechanical properties and DM status, sex, age, and body mass index (BMI). RESULTS:DM was not associated with significant differences in adipose tissue viscoelastic properties for any of the tissue types investigated. Tissue type dependent differences were however detected, with VAT having significantly lower shear storage and loss moduli than SAT and VAT ECM independent of DM status. CONCLUSION:Although DM is typically associated with adipose tissue fibrosis, it is not associated with differences in macroscopic adipose tissue mechanical properties.

中文翻译：

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糖尿病和非糖尿病人脂肪组织的粘弹性表征。

背景：肥胖引起的脂肪组织慢性炎症和纤维化促进2型糖尿病（DM）的发展。虽然已知纤维化会引起许多组织类型的机械僵硬，但尚不清楚DM是否与脂肪组织机械特性的改变有关。目的：本研究的目的是调查糖尿病是否与糖尿病（DM）和非糖尿病（NDM）肥胖受试者脂肪组织的整体粘弹性特性差异有关。方法：对肥胖的内脏（VAT）和皮下（SAT）脂肪组织进行大容量剪切流变，这些组织是从接受肥胖治疗的肥胖受试者身上采集的。还对来自脱细胞增值税（VAT ECM）的剩余细胞外基质（ECM）进行了流变学。线性混合模型用于评估脂肪组织机械特性与DM状态，性别，年龄和体重指数（BMI）之间是否存在相关性。结果：对于所研究的任何组织类型，DM与脂肪组织粘弹性特性的显着差异均不相关。但是，检测到组织类型相关的差异，与DM和状态无关，与SAT和VAT ECM相比，VAT的剪切存储和模量损失明显更低。结论：尽管糖尿病通常与脂肪组织纤维化有关，但与宏观脂肪组织机械特性的差异无关。对于所研究的任何组织类型，DM与脂肪组织粘弹性特性的显着差异均不相关。但是，检测到组织类型相关的差异，与DM和状态无关，与SAT和VAT ECM相比，VAT的剪切存储和模量损失明显更低。结论：虽然糖尿病通常与脂肪组织纤维化有关，但与宏观脂肪组织机械性能的差异无关。对于所研究的任何组织类型，DM与脂肪组织粘弹性特性的显着差异均不相关。但是，检测到组织类型相关的差异，与DM和状态无关，与SAT和VAT ECM相比，VAT的剪切存储和模量损失明显更低。结论：尽管糖尿病通常与脂肪组织纤维化有关，但与宏观脂肪组织机械特性的差异无关。