The cryobanks of agouti somatic tissues represent a promising tool for the conservation of this species and of those that are phylogenetically related and endangered. For these purposes, one strategy to guarantee the quality of samples after warming would be to choose the appropriate tissue vitrification technique. Therefore, we evaluated the effects of two different techniques, direct vitrification in cryovials (DVC) and solid-surface vitrification (SSV), on the preservation of ear somatic tissues derived from agoutis kept in a scientific center of creation. Noncryopreserved somatic tissues were used as controls. Although SSV reduced the thickness of the dermis and cartilage (p < 0.05), the epidermal thickness of these samples was observed to be similar to controls (p > 0.05). Notably, the number of fibroblasts was not altered with either technique. However, both vitrification methods led to an increase in the number of perinuclear halos, with a particularly strong increase observed in DVC-derived fragments (p < 0.05). Compared with the DVC group, SSV showed a larger number of normal chondrocytes and smaller number of degenerate chondrocytes. Furthermore, the number of empty lacunae in SSV-derived fragments remained similar to controls (p > 0.05). In summary, SSV was found to be a more efficient method for vitrifying agouti somatic tissues compared with DVC. These results are important for the proper formation of agouti somatic banks, an essential step in the study of biological resources in this species.

中文翻译：

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玻璃化技术对Agouti体细胞组织保存的影响（Dasyprocta leporina Linnaeus，1758年）。

刺足体细胞组织的冷冻库代表着一种有前途的工具，可以用于保护该物种以及与种系有关且濒临灭绝的物种。为了这些目的，一种保证加热后样品质量的策略是选择适当的组织玻璃化技术。因此，我们评估了冷冻小瓶中的直接玻璃化（DVC）和固体表面玻璃化（SSV）这两种不同技术对保存在科学创造中心中的刺豚鼠所得到的耳部体细胞组织的保护作用。未冷冻保存的体细胞组织用作对照。尽管SSV减少了真皮和软骨的厚度（p  <0.05），但观察到这些样品的表皮厚度与对照相似（p > 0.05）。值得注意的是，两种技术均未改变成纤维细胞的数量。然而，两种玻璃化方法均导致核周晕的数量增加，在DVC衍生的碎片中观察到特别强烈的增加（p  <0.05）。与DVC组相比，SSV的正常软骨细胞数量更多，而退化的软骨细胞数量更少。此外，SSV衍生片段中空腔的数量仍与对照相似（p  > 0.05）。总之，与DVC相比，SSV被发现是玻璃化刺足体组织玻璃化的更有效方法。这些结果对于正确形成刺足体细胞库是重要的，这是研究该物种生物资源的必不可少的步骤。