Multidrug-resistant (MDR) Acinetobacter baumannii is a critical threat to global health. The type strain ATCC 19606 has been widely used in studying the virulence, pathogenesis and mechanisms of antimicrobial resistance in A. baumannii. However, the lack of a complete genome sequence is a hindrance towards detailed bioinformatic studies. Here we report the generation of a complete genome for ATCC 19606 using PacBio sequencing. ATCC 19606 genome consists of a 3,980,848-bp chromosome and a 9,450-bp plasmid pMAC, and harbours a chromosomal dihydropteroate synthase gene sul2 conferring resistance to sulphonamides and a plasmid-borne ohr gene conferring resistance to peroxides. The genome also contains 69 virulence genes involved in surface adherence, biofilm formation, extracellular phospholipase, iron uptake, immune evasion and quorum sensing. Insertion sequences ISCR2 and ISAba11 are embedded in a 36.1-Kb genomic island, suggesting an IS-mediated large-scale DNA recombination. Furthermore, a genome-scale metabolic model (GSMM) iATCC19606v2 was constructed using the complete genome annotation. The model iATCC19606v2 incorporated a periplasmic compartment, 1,422 metabolites, 2,114 reactions and 1,009 genes, and a set of protein crowding constraints taking into account enzyme abundance limitation. The prediction of bacterial growth on 190 carbon and 95 nitrogen sources achieved a high accuracy of 85.6% compared to Biolog experiment results. Based upon two transposon mutant libraries of AB5075 and ATCC 17978, the predictions of essential genes reached the accuracy of 87.6% and 82.1%, respectively. Together, the complete genome sequence and high-quality GSMM iATCC19606v2 provide valuable tools for antimicrobial systems pharmacological investigations on A. baumannii.

耐多药鲍曼不动杆菌是对全球健康的重大威胁。ATCC 19606型菌株已被广泛用于研究鲍曼不动杆菌的毒力，发病机理和抗药性机制。然而，缺乏完整的基因组序列阻碍了详细的生物信息学研究。在这里，我们报告使用PacBio测序生成ATCC 19606完整基因组的过程。ATCC 19606基因组由一个3,980,848-bp的染色体和一个9,450-bp的质粒pMAC组成，并且具有赋予磺酰胺类抗性的染色体二氢蝶呤合酶基因sul2和质粒携带的ohr赋予对过氧化物抗性的基因。该基因组还包含69个毒力基因，涉及表面粘附，生物膜形成，细胞外磷脂酶，铁吸收，免疫逃逸和群体感应。插入序列IS CR2和IS Aba11嵌入36.1-Kb基因岛中，表明IS介导的大规模DNA重组。此外，使用完整的基因组注释构建了基因组规模代谢模型（GSMM）i ATCC19606v2。我的模型ATCC19606v2包含一个周质区室，1,422个代谢产物，2,114个反应和1,009个基因，以及一组考虑到酶丰度限制的蛋白质拥挤限制。与Biolog实验结果相比，在190个碳源和95个氮源上细菌生长的预测达到了85.6％的高精度。基于AB5075和ATCC 17978的两个转座子突变体文库，基本基因的预测准确率分别为87.6％和82.1％。完整的基因组序列和高质量的GSMM i ATCC19606v2共同为鲍曼不动杆菌的抗菌系统药理研究提供了有价值的工具。