Thyroid cancer is the most common endocrine malignancy in dogs. Dogs and humans are similar in the spontaneous development of thyroid cancer and metastasis to lungs; however, thyroid cancer has a higher incidence of metastasis in dogs. This study developed a preclinical nude mouse model of canine thyroid cancer using a canine thyroid adenocarcinoma cell line (CTAC) and measured the expression of important invasion and metastasis genes in spontaneous canine thyroid carcinomas and CTAC cells. CTAC cells were examined by electron microscopy. Short tandem repeat analysis was performed for both the original neoplasm and CTAC cells. CTAC cells were transduced with luciferase and injected subcutaneously and into the tail vein. Tumors and metastases were monitored using bioluminescent imaging and confirmed with gross necropsy and histopathology. Invasion and metastasis genes were characterized in 8 follicular thyroid carcinomas (FTCs), 4 C-cell thyroid carcinomas, 3 normal thyroids, and CTAC cells. CTAC cells grew well as xenografts in the subcutis, and they resembled the primary neoplasm. Metastasis to the kidney and lung occurred infrequently following subcutaneous and tail vein injection of CTAC cells. STR analysis confirmed that CTAC cells were derived from the original neoplasm and were of canine origin. Finally, 24 genes were differentially expressed in spontaneous canine thyroid carcinomas, CTAC, and normal thyroids. This study demonstrated the usefulness of a nude mouse model of experimental canine thyroid carcinoma and identified potential molecular targets of canine follicular and C-cell thyroid carcinoma.

中文翻译：

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犬甲状腺癌：裸鼠的分子特征和细胞系生长。

甲状腺癌是犬中最常见的内分泌恶性肿瘤。狗和人在甲状腺癌的自发发展和向肺的转移方面相似。然而，甲状腺癌在犬中的转移发生率较高。这项研究使用犬甲状腺腺癌细胞系（CTAC）建立了犬甲状腺癌的临床前裸鼠模型，并测量了自发性犬甲状腺癌和CTAC细胞中重要的侵袭和转移基因的表达。通过电子显微镜检查CTAC细胞。对原始肿瘤和CTAC细胞进行短串联重复分析。用萤光素酶转导CTAC细胞，皮下注射并注入尾静脉。使用生物发光成像监测肿瘤和转移，并通过大体尸检和组织病理学证实。在8个滤泡性甲状腺癌（FTC），4个C细胞甲状腺癌，3个正常甲状腺和CTAC细胞中鉴定了侵袭和转移基因。CTAC细胞在皮下异种移植中生长良好，与原发性肿瘤相似。皮下和尾静脉注射CTAC细胞后，很少发生向肾脏和肺的转移。STR分析证实CTAC细胞起源于原始肿瘤，并且是犬来源的。最后，在自发的犬甲状腺癌，CTAC和正常甲状腺中差异表达了24个基因。这项研究证明了实验性犬甲状腺癌的裸鼠模型的有用性，并确定了犬滤泡性和C细胞甲状腺癌的潜在分子靶标。