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Immunoglobulin superfamily beat-Ib mediates intestinal regeneration induced by reactive oxygen species in Drosophila.
Genes to Cells ( IF 1.3 ) Pub Date : 2020-03-10 , DOI: 10.1111/gtc.12762 Hiroki Nagai 1 , Shoichiro Kurata 1 , Tamaki Yano 1
Genes to Cells ( IF 1.3 ) Pub Date : 2020-03-10 , DOI: 10.1111/gtc.12762 Hiroki Nagai 1 , Shoichiro Kurata 1 , Tamaki Yano 1
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Reactive oxygen species (ROS) often injure intestinal epithelia that cause loss of damaged cells, which is mainly repaired by proliferation of intestinal stem cells (ISCs). To maintain the homeostatic state, coordination of sensing of the ROS injury and the subsequent epithelial cell loss with the replenishment by cell renewal is crucial. However, little is known about how gut epithelial cells initiate regenerative responses against ROS to maintain the tissue integrity. Here, we carried out a genome-wide screen, by which we identify immunoglobulin superfamily beaten path Ib (beat-Ib) as an essential gene for provoking ISC proliferation against ROS in Drosophila intestine. Interestingly, the beat-Ib function is required in differentiated enterocytes, the main targeted cells by ROS in the intestinal tract, but is dispensable in the stem cells. Moreover, beat-Ib is not involved in enterocyte apoptosis at ROS injury. These findings indicate the essential role of beat-Ib in Drosophila midgut enterocytes for initiating the non-cell-autonomous induction of ISC division in response to environmental ROS stresses.
中文翻译：
免疫球蛋白超家族beat-Ib介导果蝇中活性氧诱导的肠道再生。
活性氧（ROS）通常会损伤肠上皮，从而导致受损细胞的丢失，这主要是通过肠干细胞（ISC）的增殖来修复的。为了保持体内稳态，对ROS损伤和随后的上皮细胞丢失的感知与通过细胞更新进行补充的协调至关重要。然而，关于肠上皮细胞如何启动针对ROS的再生反应以维持组织完整性的了解甚少。在这里，我们进行了全基因组筛选，通过该筛选，我们将免疫球蛋白超家族殴打路径Ib（beat-Ib）确定为在果蝇肠道中引起ISC对抗ROS增殖的必需基因。有趣的是，在分化的肠细胞中，beat-Ib功能是必需的，肠细胞是ROS的主要靶向细胞，但在干细胞中却是必需的。而且，beat-Ib不参与ROS损伤时的肠细胞凋亡。这些发现表明，果蝇中肠肠上皮细胞中的beat-Ib对于启动对环境ROS胁迫的ISC分裂的非细胞自主诱导作用至关重要。
更新日期：2020-03-10
中文翻译：
免疫球蛋白超家族beat-Ib介导果蝇中活性氧诱导的肠道再生。
活性氧（ROS）通常会损伤肠上皮，从而导致受损细胞的丢失，这主要是通过肠干细胞（ISC）的增殖来修复的。为了保持体内稳态，对ROS损伤和随后的上皮细胞丢失的感知与通过细胞更新进行补充的协调至关重要。然而，关于肠上皮细胞如何启动针对ROS的再生反应以维持组织完整性的了解甚少。在这里，我们进行了全基因组筛选，通过该筛选，我们将免疫球蛋白超家族殴打路径Ib（beat-Ib）确定为在果蝇肠道中引起ISC对抗ROS增殖的必需基因。有趣的是，在分化的肠细胞中，beat-Ib功能是必需的，肠细胞是ROS的主要靶向细胞，但在干细胞中却是必需的。而且，beat-Ib不参与ROS损伤时的肠细胞凋亡。这些发现表明，果蝇中肠肠上皮细胞中的beat-Ib对于启动对环境ROS胁迫的ISC分裂的非细胞自主诱导作用至关重要。
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