Outbreaks of diseases in farmed fish remain a recurring problem despite the development of vaccines and improved hygiene standards on aquaculture farms. One commonly observed bacterial disease in tropical aquaculture of the South-East Asian region is tenacibaculosis, which is attributed to members of the genus Tenacibaculum (family Flavobacteriaceae, phylum Bacteroidetes), most notably Tenacibaculum maritimum. The impact of tenacibaculosis on the fish microbiota remains poorly understood. In this study, we analysed the microbiota of different tissues of commercially reared Asian seabass (Lates calcarifer) that showed symptoms of tenacibaculosis and compared the microbial communities to those of healthy and experimentally infected fish that were exposed to diseased farmed fish. The relative abundance of Tenacibaculum species in experimentally infected fish was significantly lower than in commercially reared diseased fish and revealed a higher prevalence of different Tenacibaculum species. One isolated strain, TLL-A2T, shares 98.7% 16S rRNA gene identity with Tenacibaculum mesophilum DSM 13764T. The genome of strain TLL-A2T was sequenced and compared to that of T. mesophilum DSM 13764T. Analysis of average nucleotide identity and comparative genome analysis revealed only 92% identity between T. mesophilum DSM 13764T and strain TLL-A2T and differences between the two strains in predicted carbohydrate activating enzymes respectively. Phenotypic comparison between strain TLL-A2T and T. mesophilum DSM 13764T indicated additional differences, such as growth response at different salt concentrations. Based on molecular and phenotypic differences, strain TLL-A2T (=DSM 106434T, KCTC 62393T) is proposed as the type strain of Tenacibaculum singaporense sp. nov.

中文翻译：

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深入了解养殖的亚洲鲈鱼（鳞片鳞茎）的微生物组，该症状带有腱鞘杆菌病和对新加坡腱鞘菌的描述。十一月

尽管已经开发了疫苗并改善了水产养殖场的卫生标准，但养殖鱼的疾病暴发仍然是一个经常发生的问题。东南亚地区热带水产养殖中最常观察到的细菌病是藤杆菌，这归因于藤杆菌属（黄杆菌科，拟杆菌门属）的成员，最显着的是海藤杆菌。藤杆菌对鱼类微生物群的影响仍然知之甚少。在这项研究中，我们分析了商业养殖的亚洲鲈鱼（Lates calcarifer）不同组织的微生物，这些微生物表现出腱生病的症状，并将微生物群落与暴露于患病养殖鱼类的健康和实验感染鱼类的微生物群落进行了比较。经试验感染的鱼类中的Tenacibaculum种类的相对丰度显着低于商业养殖的患病鱼类，并且显示出不同Tenacibaculum种类的患病率较高。一种分离的菌株，TLL-A2T，与嗜中生Tenacibaculum mesophilum DSM 13764T具有98.7％的16S rRNA基因同一性。对菌株TLL-A2T的基因组进行测序，并与嗜麦草DSM 13764T的基因组进行比较。平均核苷酸同一性的分析和比较基因组分析显示，嗜温红球菌DSM 13764T与菌株TLL-A2T之间仅具有92％的同一性，并且两种菌株之间的差异分别位于预测的碳水化合物激活酶中。TLL-A2T菌株和嗜温热单胞菌DSM 13764T之间的表型比较表明存在其他差异，例如在不同盐浓度下的生长反应。根据分子和表型差异，建议将菌株TLL-A2T（= DSM 106434T，KCTC 62393T）选作新加坡担架杆菌（Tenacibaculum singaporense sp。）的类型菌株。十一月