Teleosts have the ability to regenerate their caudal fin upon amputation. A highly proliferative mass of undifferentiated cells called blastema forms beneath wound epidermis and differentiates to regenerate all missing parts of the fin. To date, the origin and fate of the blastema is not completely understood. However, current hypotheses suggest that the blastema is comprised of lineage-restricted dedifferentiated cells. To investigate the differentiation capacity of regenerating fin-derived cells, primary cultures were initiated from the explants of 2-days post-amputation (dpa) regenerates of juvenile gilthead seabream (Sparus aurata). These cells were subcultured for over 30 passages and were named as BSa2. After 10 passages they were characterized for their ability to differentiate towards different bone cell lineages and mineralize their extracellular matrix, through immunocytochemistry, histology, and RT-PCR. Exogenous DNA was efficiently delivered into these cells by nucleofection. Assessment of lineage-specific markers revealed that BSa2 cells were capable of osteo/chondroblastic differentiation. BSa2 cells were also found to be capable of osteoclastic differentiation, as demonstrated through TRAP-specific staining and pit resorption assay. Here, we describe the development of the first successful cell line viz., BSa2, from S. aurata 2-dpa regenerating caudal fins, which has the ability of multilineage differentiation and is capable of in vitro mineralization. The availability of such in vitro cell systems has the potential to stimulate research on the mechanisms of cell differentiation during fin regeneration and provide new insights into the mechanisms of bone formation.

中文翻译：

---

从再生金头尾鳍分离的细胞可以分化为不同的骨细胞谱系。

硬骨鱼有截肢时再生其尾鳍的能力。在伤口表皮下形成称为胚细胞的未分化细胞的大量增殖，并分化以再生鳍的所有缺失部分。迄今为止，胚泡的起源和命运尚不完全清楚。但是，目前的假设表明胚细胞由谱系限制的去分化细胞组成。调查再生鳍来源的细胞的分化能力，少年金头鲷（的原代培养物从2天，将外植启动后的截肢（DPA）回收物金头鲷）。将这些细胞传代培养超过30代，并命名为BSa2。经过10代传代后，他们通过免疫细胞化学，组织学和RT-PCR鉴定了向不同骨细胞谱系分化并使其细胞外基质矿化的能力。通过核转染将外源DNA有效地递送到这些细胞中。谱系特异性标记的评估表明，BSa2细胞能够分化成骨/软骨细胞。通过TRAP特异性染色和凹坑吸收试验证明，BSa2细胞也具有破骨细胞分化的能力。在这里，我们描述了来自金黄色葡萄球菌的第一个成功的细胞系BSa2的开发。2-dpa再生尾鳍，具有多谱系分化能力并且能够体外矿化。这种体外细胞系统的可用性有可能刺激鳍鳍再生过程中细胞分化机制的研究，并为骨形成机制提供新的见解。