Over a 3-year-period, 17 wild-caught opaleye (Girella nigricans) housed in a public display aquarium were found dead without premonitory signs. Grossly, 4 animals had pinpoint brown or black foci on coelomic adipose tissue. Histologically, liver, spleen, heart, and posterior kidney had mesomycetozoan granulomas in all cases; other organs were less commonly infected. Four opaleye had goiter; additional substantial lesions were not identified. Granulomas surrounded melanized debris, leukocytes, and mesomycetozoa represented by folded membranes (collapsed schizont walls), intact schizonts (50- to >200 µm in diameter with a multilaminate membrane), plasmodia (budding from schizonts or free in tissue), or rarely germinal tubes (budding from schizonts). Ichthyophonus was grown from fresh tissues in tissue explant broth cultures of the heart, liver, and/or spleen. Polymerase chain reaction using 18S ribosomal DNA primers amplified a 1730-bp region, and the DNA sequence was most similar to Ichthyophonus hoferi, which is often associated with freshwater aquaculture fish.

中文翻译：

---

鱼鳞藻 Opaleye（Girella nigricans）中的感染。

超过3年的时间，发现在公共展示水族馆中饲养的17只野性欧泊眼（Girella nigricans）死亡，没有任何先兆迹象。总体上，有4只动物在结肠脂肪组织上发现了棕色或黑色病灶。从组织学上讲，所有情况下，肝，脾，心脏和肾后部均患有中生动物肉芽肿。其他器官感染较少。四个蛋白石有甲状腺肿。未发现其他实质性病变。肉芽肿围绕着黑色素化的碎片，白细胞和中生动物，以折叠的膜（塌陷的裂殖体壁），完整的裂殖体（直径为50-> 200 µm，带有多层膜），浆原体（裂殖体发芽或组织游离）或很少生发为代表管（裂片芽）。鱼腥草是从心脏，肝脏，和/或脾脏。使用18S核糖体DNA引物进行的聚合酶链式反应扩增了一个1730 bp的区域，该DNA序列与霍夫鱼白痢鱼（Ichthyophonus hoferi）最相似，后者通常与淡水养殖鱼类有关。