Abstract

Coat color is one of the major quality traits of animals, and miR-129-5p acts as an important regulator for melanin biosynthesis in mammals. In this study, real-time PCR and western blotting were used to examine the expression of miR-129-5p and its targets genes in the skin of different coat color goats. The results showed that the expression of miR-129-5p in the skin samples of Inner Mongolia cashmere goats (IMCG) was higher than that of Dazu black goat (DBG). Also, the target genes (tyrosinase (TYR), frizzled 6 (FZD6) and glycogen synthase kinase 3β (GSK3β)) of miR-129-5p was highly expressed in the skin samples of DBG. The expression of miR-129-5p firstly increased and then decreased with age in F1 hybrid generation of DBG and IMCG. In addition, the expression of TYR decreased with age, while the expression of MITF increased with age but then decreased. The expression of FZD6 and GSK3β in the skin samples of F1 of different ages were irregular. Our results indicated that miR-129-5p mainly affects the formation of coat color of goats by decreasing the expression of TYR. This study suggests that miR-129-5p can act as a suppressor in the formation of coat color to lay the foundation for studying the effect of miR-129-5p on melanin synthesis.

摘要

毛色是动物的主要品质特征之一，miR-129-5p 是哺乳动物黑色素生物合成的重要调节因子。本研究采用实时荧光定量 PCR 和蛋白质印迹法检测 miR-129-5p 及其靶基因在不同毛色山羊皮肤中的表达。结果表明，内蒙古绒山羊（IMCG）皮肤样品中miR-129-5p的表达高于大足黑山羊（DBG）。此外，靶基因（酪氨酸酶 ( TYR )、卷曲 6 ( FZD6 ) 和糖原合酶激酶 3β ( GSK3β))) 的 miR-129-5p 在 DBG 的皮肤样本中高度表达。miR-129-5p的表达在DBG和IMCG的F1杂种世代中随着年龄的增长先升高后降低。此外，TYR的表达随着年龄的增长而下降，而MITF的表达随着年龄的增长而增加然后下降。FZD6和GSK3β在不同年龄F1皮肤样本中的表达不规则。我们的研究结果表明，miR-129-5p 主要通过降低TYR的表达来影响山羊毛色的形成。本研究表明miR-129-5p可以作为毛色形成的抑制剂，为研究miR-129-5p对黑色素合成的影响奠定基础。