BACKGROUND This study aimed to investigate the clinical characteristics of miR-450b-3p in the patients of gastric cancer (GC), and further explore whether miR-450b-3p could inhibit the proliferation of GC cells via regulating KLF7. METHODS Real-time quantitative PCR (qRT-PCR) was performed to detect the expression level of miR-450b-3p in 48 GC patients of tumor tissue and paracancerous tissue specimens collected, and the associations between miR-450b-3p and the clinical characteristics of GC patients were analyzed. Meanwhile, the expression of miR-450b-3p in GC cell lines was verified using qRT-PCR. miR-450b-3p overexpression vectors was constructed in GC cell lines including AGS and BGC-823, and then CCK-8 cell proliferation assay, Plate colony formation assay and EdU assay were applied to analyze the biological function of miR-450b-3p in GC cell lines. RESULTS The results of qRT-PCR showed that the expression level of miR-450b-3p in GC tissues was lower than that in paracancerous tissues, and the difference was statistically significant. Compared with GC patients with high-miR-450b-3p expression, these GC patients with low-miR-450b-3p expression had a higher pathological stage and tumor size. Subsequently, the proliferation ability of GC cells in miR-450b-3p mimic was significantly decreased when comparing with the NC mimic. In addition, qRT-PCR indicated that the expression level of KLF7 significantly decreased after miR-450b-3p mimic. Therefore, it was demonstrated that miR-450b-3p might inhibit the malignant progression of GC via modulating KLF7. Bioinformatics analysis and dual luciferase reporter suggested miR-450b-3p was bound to KLF7. Finally, the results of the reverse experiment confirmed that overexpression of KLF7 could reverse miR-450b-3p mimic induced-inhibition of GC malignant progression. CONCLUSIONS Generally, miR-450b-3p significantly down-regulated in GC tissues and cell lines, and was associated with the pathological stage and tumor size of GC patients. Meanwhile, miR-450b-3p inhibited cell proliferation in GC via modulating KLF7.

中文翻译：

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miR-450b-3p通过调节KLF7抑制胃癌的增殖。

背景本研究旨在探讨miR-450b-3p在胃癌（GC）患者中的临床特征，进一步探讨miR-450b-3p是否能通过调节KLF7抑制胃癌细胞增殖。方法采用实时定量PCR（qRT-PCR）检测48例GC患者的肿瘤组织和癌旁组织标本中miR-450b-3p的表达水平，以及miR-450b-3p与临床特征的关系。 GC 患者进行了分析。同时，使用qRT-PCR验证了miR-450b-3p在GC细胞系中的表达。在包括AGS和BGC-823在内的GC细胞系中构建miR-450b-3p过表达载体，然后进行CCK-8细胞增殖实验，应用平板集落形成试验和EdU试验分析miR-450b-3p在GC细胞系中的生物学功能。结果qRT-PCR结果显示，miR-450b-3p在GC组织中的表达水平低于癌旁组织，差异有统计学意义。与具有高miR-450b-3p表达的GC患者相比，这些具有低miR-450b-3p表达的GC患者具有更高的病理分期和肿瘤大小。随后，与NC模拟相比，miR-450b-3p模拟中GC细胞的增殖能力显着降低。此外，qRT-PCR 表明 KLF7 的表达水平在 miR-450b-3p 模拟后显着降低。因此，证明 miR-450b-3p 可能通过调节 KLF7 抑制 GC 的恶性进展。生物信息学分析和双荧光素酶报告表明 miR-450b-3p 与 KLF7 结合。最后，反向实验的结果证实了KLF7的过表达可以逆转miR-450b-3p模拟诱导的GC恶性进展的抑制作用。结论 一般而言，miR-450b-3p在GC组织和细胞系中显着下调，并且与GC患者的病理分期和肿瘤大小有关。同时，miR-450b-3p通过调节KLF7抑制GC中的细胞增殖。并且与GC患者的病理分期和肿瘤大小有关。同时，miR-450b-3p通过调节KLF7抑制GC中的细胞增殖。并且与GC患者的病理分期和肿瘤大小有关。同时，miR-450b-3p通过调节KLF7抑制GC中的细胞增殖。