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Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices
Helminthologia ( IF 1 ) Pub Date : 2020-01-25 , DOI: 10.2478/helm-2020-0010
S J Hammad 1 , S Cavallero 2 , F S Al-Nasiri 1 , S D Amelio 2
Affiliation  

Summary Echinococcus granulosus is a tapeworm whose life cycle includes dogs and other canines as final hosts, while domestic and wild ungulates act as intermediate hosts for the tissue-invading larval stage (metacestode). E. granulosus has a worldwide geographical distribution. Protoscolices and rostellar hooks of E. granulosus are useful for diagnosis and rostellar hook morphometric features may be useful to discriminate E. granulosus and related species. The present study was aimed to determine a more suitable lytic solution and to obtain a clearest vision for performing morphometric studies on the rostellar hooks of E. granulosus protoscolices. Five fertile hydatid cyst samples were collected from sheep in Kirkuk slaughterhouse, Iraq, during June of 2015. According to the results of the present study, proteinase-K lytic solution is the best approach in morphometric analysis to get a clear vision of rostellar hooks and a safer usage in comparison with solutions containing lactophenol (lactophenol, lactophenol blue).

中文翻译:

乳酚蓝、乳酚溶液和蛋白酶-K 裂解液对细粒棘球绦虫的喙状钩形态测定的比较研究

总结细粒棘球绦虫是一种绦虫,其生命周期包括狗和其他犬科动物作为最终宿主,而家养和野生有蹄类动物则作为侵入组织的幼虫阶段(毛绦虫)的中间宿主。E. granulosus 具有世界范围的地理分布。E. granulosus 的 Protoscolices 和 rostellar hooks 可用于诊断,并且 rostellar hook 形态测量特征可能有助于区分 E. granulosus 和相关物种。本研究旨在确定更合适的裂解溶液,并获得对 E. granulosus protoscolices 的 rostellar 钩进行形态测量研究的最清晰的愿景。2015 年 6 月,从伊拉克基尔库克屠宰场的绵羊身上采集了 5 个可育的包虫包囊样本。 根据本研究的结果,
更新日期:2020-01-25
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