The Chinese chestnut (Castanea mollissima Blume) 'Huaihuang' was chosen as the experimental material to observe embryogenesis and the dynamic changes of cell wall components during this process. Various developmental stages of embryos, including globular embryos, heart embryos, torpedo embryos and cotyledon embryos, were observed. The results showed that during embryogenesis, cellulose increased, and callose rapidly degraded. In the cell walls of developing embryos, pectic homogalacturonan (HG), especially low-esterified HG, was abundant, suggesting rapid synthesis and de-methyl-esterification of HG. Extensin and galactan increased with the development of the embryos. In contrast, the arabinan epitopes decreased in developing embryos but were more abundant than galactan epitopes at all stages. Xylan epitopes showed explicit boundaries between the outer epidermal wall and the rest of the inner tissues, and the fluorescence intensity of the outer epidermal wall was significantly higher than that of the inner tissues. Furthermore, the results indicated that the outer epidermal wall contained high amounts of cellulose, HG pectin and hemicellulose, especially arabinan and xylan. These results suggested the presence of rapid pectin metabolism, cellulose synthesis, rapid degradation of callose, different distributive patterns and dynamic changes of hemicellulose (galactan, arabinan and xylan) and extensin during embryogenesis. Various cell wall components exist in different tissues of the embryo, and dynamic changes in cell wall components are involved in the embryonic development process.

中文翻译：

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板栗胚发生过程中细胞壁成分的变化。

选择板栗（Castanea mollissima Blume）“槐黄”作为观察该过程中胚发生和细胞壁成分动态变化的实验材料。观察到胚的各个发育阶段，包括球形胚，心胚，鱼雷胚和子叶胚。结果表明，在胚胎发生过程中，纤维素增加，并且call愈迅速降解。在发育中的胚胎的细胞壁中，果胶高半乳糖醛酸（HG），尤其是低酯化的HG丰富，表明HG的快速合成和去甲基酯化。延伸蛋白和半乳聚糖随着胚胎的发育而增加。相反，阿拉伯糖蛋白表位在发育中的胚胎中减少，但在各个阶段均比半乳聚糖表位丰富。木聚糖表位在表皮外壁和其余内部组织之间显示出明确的边界，并且表皮外壁的荧光强度显着高于内部组织。此外，结果表明表皮外壁含有大量纤维素，HG果胶和半纤维素，尤其是阿拉伯聚糖和木聚糖。这些结果表明在胚发生过程中存在快速的果胶代谢，纤维素合成，call质的快速降解，不同的分布模式以及半纤维素（半乳聚糖，阿拉伯聚糖和木聚糖）和延伸蛋白的动态变化。胚胎的不同组织中存在各种细胞壁成分，并且细胞壁成分的动态变化参与胚胎发育过程。