In this review, we discuss methods and advancements in fluorescence lifetime imaging microscopy that permit measurements to be performed at faster speed and higher resolution than previously possible. We review fast single-photon timing technologies and the use of parallelized detection schemes to enable high-throughput and high content imaging applications. We appraise different technological implementations of fluorescence lifetime imaging, primarily in the time-domain. We also review combinations of fluorescence lifetime with other imaging modalities to capture multi-dimensional and correlative information from a single sample. Throughout the review, we focus on applications in biomedical research. We conclude with a critical outlook on current challenges and future opportunities in this rapidly developing field.

中文翻译：

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高通量、多参数、相关荧光寿命成像。


在这篇综述中，我们讨论了荧光寿命成像显微镜的方法和进步，这些方法和进步允许以比以前更快的速度和更高的分辨率进行测量。我们回顾了快速单光子定时技术以及并行检测方案的使用，以实现高通量和高内容成像应用。我们评估荧光寿命成像的不同技术实现，主要是在时域。我们还回顾了荧光寿命与其他成像方式的组合，以从单个样品中捕获多维和相关信息。在整个审查过程中，我们重点关注生物医学研究中的应用。最后，我们对这个快速发展的领域当前的挑战和未来的机遇进行了批判性的展望。