The inflammation of chronic wounds generally causes delaying their healing process. The present work aims to formulate a wound dressing polyelectrolyte membrane based on chitosan (Ch) and sodium hyaluronate (HA) loaded with glutathione (GSH). The membrane types (Ch/HA and Ch/HA/GSH) were examined by Fourier transform infrared spectroscopy (FT-IR). The material properties were further investigated using thermal gravimetric analysis (TGA) and scanning electron microscopy (SEM). Physical characteristics of the prepared membranes, such as wettability, surface roughness, and mechanical properties were determined by standard experimental methods. In vitro assays were used to evaluate the haemocompatibility, thrombogenicity, and cytotoxicity of the membranes. The wound healing examined using a standard rat model exhibited a progress at exploiting the Ch/HA/GSH-type membranes compared to a bicomponent Ch/HA membrane or a "dry" healing wound. Histological examination of the recovered skin confirmed the visual observations. In conclusion, in vivo study results assert that Ch/HA/GSH is a proper wound-dressing for healing the chronic skin wounds.

中文翻译：

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负载谷胱甘肽的壳聚糖/透明质酸聚电解质膜增强伤口愈合：体外和体内评价。

慢性伤口的炎症通常会导致其愈合过程延迟。本工作旨在基于载有谷胱甘肽（GSH）的壳聚糖（Ch）和透明质酸钠（HA）配制伤口敷料聚电解质膜。膜类型（Ch / HA和Ch / HA / GSH）通过傅立叶变换红外光谱（FT-IR）进行检查。使用热重分析（TGA）和扫描电子显微镜（SEM）进一步研究了材料性能。通过标准实验方法确定所制备的膜的物理特性，例如润湿性，表面粗糙度和机械性能。体外测定用于评估膜的血液相容性，血栓形成性和细胞毒性。与双组分Ch / HA膜或“干”愈合伤口相比，使用标准大鼠模型检查的伤口愈合在利用Ch / HA / GSH型膜方面表现出进步。对恢复的皮肤进行组织学检查证实了视觉观察。总之，体内研究结果断言Ch / HA / GSH是治疗慢性皮肤伤口的合适伤口敷料。