Some Rhodotorula spp. have been characterized as oleaginous yeasts. Under certain culture conditions they can accumulate neutral lipids, which are mainly triglycerides (TAG). Microbial TAG that can be used as raw material for biodiesel synthesis are attractive for the biofuel industry. In this study, the ability to synthesize lipids of Rhodotorula glutinis R4, isolated in Antarctica, was compared with eight strains belonging to the genera Rhodotorula and Yarrowia with the aim of proposing a novel source of oils for biodiesel synthesis. All strains were cultured under nitrogen (N) limiting conditions and an excess of carbon (C) in the culture medium. We found that yeasts accumulated between 9-48.9 % (w/w) of lipids. Among them, R. glutinis R4 showed the highest growth (14 g L-1, μmax 0,092 h-1) and lipid production (7 g L-1; 47 % w/w). Microbial oils produced by R. glutinis R4 are similar to vegetable oils, with 61 % of oleic acid, indicating that it is adequate for biodiesel synthesis. Our results demonstrate that biodiesel derived from R. glutinis R4 complies with international fuel standards ASTM D6751 and EN 14214. Therefore, this work demonstrates that Rhodotorula glutinis R4 is a novel and valuable source of microbial oils for biodiesel synthesis.

中文翻译：

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与其他含油酵母相比，Rhodotorula glutinis R4的生长和脂质产生。

一些红景天属。已经被表征为油质酵母。在某些培养条件下，它们会积聚中性脂质，主要是甘油三酸酯（TAG）。可用作生物柴油合成原料的微生物TAG对生物燃料行业具有吸引力。在这项研究中，将南极分离的Rhodotorula glutinis R4脂质的合成能力与属于Rhodotorula和Yarrowia属的八种菌株进行了比较，目的是提出一种新的生物柴油合成油来源。将所有菌株在氮（N）限制条件和培养基中过量的碳（C）下培养。我们发现酵母积累了9-48.9％（w / w）的脂质。其中，谷胱甘肽R4显示最高的生长（14 g L-1，μmax0,092 h-1）和脂质产生（7 g L-1； 47％w / w）。谷氨酸棒杆菌R4产生的微生物油与植物油相似，具有61％的油酸，表明它足以用于生物柴油的合成。我们的结果表明，源自R. glutinis R4的生物柴油符合国际燃料标准ASTM D6751和EN14214。因此，这项工作表明，Rhodotorula glutinis R4是用于生物柴油合成的新型且有价值的微生物油来源。