During the early 1900s, Northern chamois ( Rupicapra rupicapra ) populations in the northern Dinaric Mountains were extirpated. During the 1960s and 1970s there were several reintroductions of individuals from two Northern chamois subspecies (Alpine chamois, R. r. rupicapra and Balkan chamois, R. r. balcanica ) from neighbouring areas in the attempt to re-establish the population. Accurate taxonomic classification, at subspecies level, of the autochthonous extirpated population was not known. To clarify which subspecies was present before reintroduction, we genotyped four male chamois skulls originating from Velebit Mountain, collected around 25 years before the population local extinction. DNA was successfully extracted from middle layer and outer sheath of horns. Assignment based on microsatellite loci, using both Bayesian clustering in STRUCTURE (with q values between 0.55 and 0.73) and DAPC (with individual membership probabilities of 0.99 and 1.00) indicated higher assessed likelihood for the Alpine subspecies.

中文翻译：

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基于微卫星的任务揭示了已灭绝的山地有蹄类动物的历史

在 1900 年代初期，迪那利克山脉北部的北部羚羊 (Rupicapra rupicapra) 种群被灭绝。在 1960 年代和 1970 年代，从邻近地区重新引入了两个北方羚羊亚种（高山羚羊，R. r. rupicapra 和巴尔干羚羊，R. r. balcanica）的个体，试图重新建立种群。在亚种水平上，本地灭绝种群的准确分类学分类尚不清楚。为了阐明在重新引入之前存在哪些亚种，我们对来自 Velebit 山的四个雄性麂皮头骨进行了基因分型，这些头骨是在种群局部灭绝前大约 25 年收集的。成功从角的中层和外鞘中提取DNA。基于微卫星位点的分配，