The Pestivirus genus comprises species that affect animal health and productivity worldwide. Members of the Suidae family are hosts for classical swine fever virus (CSFV), an important pathogen tracked by the World Organization for Animal Health (OIE). However, swine are also susceptible to other pestivirus species that can result in disease or compromise CSFV detection. We searched for pestivirus infection in swine sera collected from 320 backyard pig herds in southern Brazil. We used reverse-transcription PCR primers for Bungowannah virus; atypical porcine pestivirus (APPV); and a panpestivirus pair that detects bovine viral diarrhea virus (BVDV)-1, -2, and HoBi-like pestivirus (HoBiPeV), border disease virus (BDV), and CSFV. Two samples were positive using the panpestivirus primer pair and were classified as BVDV-1d and -2a, respectively. Serum samples were tested for virus neutralization against BVDV-1a, -1b, and -2 strains, resulting in 28 (4.4%) positive samples. Of those, 16 samples had the highest titers against BVDV-1a (2), BVDV-1b (5), and BVDV-2 (9). Our results indicate that Bungowannah virus, APPV, CSFV, BDV, and HoBiPeV have not been circulating in these specific backyard swine populations. However, ruminant pestiviruses were detected and must be considered in future pestivirus control programs conducted in Brazil.

中文翻译：

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巴西南部后院猪瘟病毒调查。

瘟病毒属包括影响全世界动物健康和生产力的物种。Suidae家族的成员是经典猪瘟病毒（CSFV）的宿主，经典猪瘟病毒是世界动物卫生组织（OIE）追踪的重要病原体。但是，猪也易感染其他瘟病毒，可能导致疾病或损害CSFV检测。我们在从巴西南部的320个后院猪群收集的猪血清中搜索瘟病毒感染。我们使用了Bungowannah病毒的逆转录PCR引物；非典型猪瘟病毒（APPV）; 以及检测出牛病毒性腹泻病毒（BVDV）-1，-2和HoBi样瘟病毒（HoBiPeV），边界病病毒（BDV）和CSFV的全瘟病毒对。使用全瘟病毒引物对获得的两个样本均为阳性，分别分类为BVDV-1d和-2a。测试血清样品对BVDV-1a，-1b和-2菌株的病毒中和作用，得到28个（4.4％）阳性样品。在这些样品中，有16个样品对BVDV-1a（2），BVDV-1b（5）和BVDV-2（9）的滴度最高。我们的结果表明，在这些特定的后院猪种群中，Bungowannah病毒，APPV，CSFV，BDV和HoBiPeV尚未传播。但是，发现了反刍动物瘟病毒，必须在巴西今后进行的瘟病毒控制计划中予以考虑。