Addressing the complexity of organogenesis at a system-wide level requires a complete understanding of adult cell types, their origin, and precursor relationships. The Drosophila ovary has been a model to study how coordinated stem cell units, germline, and somatic follicle stem cells maintain and renew an organ. However, lack of cell type-specific tools have limited our ability to study the origin of individual cell types and stem cell units. Here, we used a single-cell RNA sequencing approach to uncover all known cell types of the developing ovary, reveal transcriptional signatures, and identify cell type-specific markers for lineage tracing. Our study identifies a novel cell type corresponding to the elusive follicle stem cell precursors and predicts subtypes of known cell types. Altogether, we reveal a previously unanticipated complexity of the developing ovary and provide a comprehensive resource for the systematic analysis of ovary morphogenesis.

中文翻译：

---

发育中的果蝇卵巢的单细胞图谱鉴定了卵泡干细胞祖细胞。


在全系统水平上解决器官发生的复杂性需要完全了解成体细胞类型、它们的起源和前体关系。果蝇卵巢是研究协调干细胞单位、种系和体细胞卵泡干细胞如何维持和更新器官的模型。然而，缺乏细胞类型特异性工具限制了我们研究个体细胞类型和干细胞单位起源的能力。在这里，我们使用单细胞 RNA 测序方法来揭示发育中卵巢的所有已知细胞类型，揭示转录特征，并识别用于谱系追踪的细胞类型特异性标记。我们的研究确定了一种与难以捉摸的卵泡干细胞前体相对应的新细胞类型，并预测了已知细胞类型的亚型。总而言之，我们揭示了卵巢发育中以前未预料到的复杂性，并为卵巢形态发生的系统分析提供了全面的资源。