The phelligridin LA was one of the valuable metabolites synthesized by the medicinal fungus Sanghuang in liquid fermentation. In the improvement of PLA productivity by fermentation, we investigated the optimal conditions for the efficient separation of PLA from the fermentation broth with a chromatographic column packed with the macroporous resin ADS-17. Based on the findings, we further developed an integrated bioreactor system that coupled the fermentation and separation of PLA. Fermentation experiments with the bioreactor system testified the performance of our design in fortification of the PLA production: an improvement of PLA production by 2.14 folds was successfully achieved due to the prompt removal of the PLA, while the formation of hyphae biomass was not affected. Also, the integrated system could afford a simultaneous purification of PLA to a purity of 92.95% with a recovery of 84.3%, which was comparable to that of the PLA purified with an additional process (97.53%), at a reasonable recovery. This study provided a feasible approach for the improved production of PLA by fermentation. Besides, the design of the integrated bioreactor system offered a useful reference for the fermentation process development of fungi for the production of diverse valuable metabolites.

中文翻译：

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Inonotus baumii通过整合的发酵-分离工艺高效生产了Phelligridin LA。

水飞蓟素LA是药用菌桑黄在液体发酵中合成的有价值的代谢产物之一。在通过发酵提高PLA生产率的过程中，我们使用装有大孔树脂ADS-17的色谱柱研究了从发酵液中有效分离PLA的最佳条件。基于这些发现，我们进一步开发了一个集成的生物反应器系统，该系统结合了PLA的发酵和分离。使用生物反应器系统进行的发酵实验证明了我们设计在强化PLA生产中的性能：由于PLA的迅速去除，PLA产量成功提高了2.14倍，而菌丝生物质的形成并未受到影响。也，该集成系统可以同时纯化PLA达到92.95％的纯度，回收率84.3％，与通过合理的回收率通过附加工艺纯化的PLA的纯度（97.53％）相当。该研究为通过发酵提高PLA产量提供了可行的方法。此外，集成生物反应器系统的设计为真菌发酵过程的发展提供了有用的参考，以生产各种有价值的代谢产物。