Selenium nanoparticles (Se NPs) were synthesized using Saccharomyces cerevisiae yeast. Influences of different amounts of sodium selenite (5.0, 10.0, 15.0, 20.0, and 25 µg) were evaluated on growth of yeast during incubation at 32 °C, during 4 days. UV–Vis spectroscopy results have shown that synthesized Se NPs had broad emission peak (λmax) in the wavelength around 350 nm which demonstrated that formation of Se NPs occurred in intracellular manner. Physico-chemical characteristics of the synthesized Se NPs using dynamic light scattering particle-size analyzer indicated that the fabricated Se NPs had particle size, polydispersity index, and zeta potential ranging from 75 to 709 nm, 0.189 to 0989, and −7.06 to −10.3 mV, respectively. Obtained results revealed that intracellular Se NPs with minimum particle size (75 nm), maximum zeta potential (−10.3 mV), and antioxidant activity (48.5%) were synthesized using minimum amount of selenium salt (5 µg). However, most uniform Se NPs were formed using maximum amount of selenium salt (25 µg). Results also indicated that by increasing amount of sodium selenite in the culture media, from 5.0 to 25 µg, antioxidant activity of the formed Se NPs decreased from 48.5 to 20.8, respectively.

中文翻译：

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使用酿酒酵母合成细胞内硒纳米颗粒的纳米生物技术方法——制造和表征

硒纳米颗粒 (Se NPs) 是使用酿酒酵母合成的。在 4 天的 32 °C 下，评估了不同量的亚硒酸钠（5.0、10.0、15.0、20.0 和 25 µg）对酵母生长的影响。UV-Vis 光谱结果表明合成的 Se NPs 在 350 nm 附近具有宽发射峰 (λmax)，这表明 Se NPs 的形成以细胞内方式发生。使用动态光散射粒度分析仪合成的 Se NPs 的物理化学特性表明，制备的 Se NPs 的粒径、多分散指数和 zeta 电位范围为 75 至 709 nm、0.189 至 0989 和 -7.06 至 -10.3 mV，分别。获得的结果表明，细胞内硒纳米颗粒具有最小粒径 (75 nm)、最大 zeta 电位 (-10. 3 mV) 和抗氧化活性 (48.5%) 是使用最少量的硒盐 (5 µg) 合成的。然而，大多数均匀的硒纳米颗粒是使用最大量的硒盐 (25 µg) 形成的。结果还表明，通过增加培养基中亚硒酸钠的量，从 5.0 到 25 µg，形成的硒纳米颗粒的抗氧化活性分别从 48.5 降低到 20.8。