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Simultaneous multielement analysis by ICP-MS with simple whole blood sample dilution and its application to uremic patients undergoing long-term hemodialysis.
Scandinavian Journal of Clinical and Laboratory Investigation ( IF 1.3 ) Pub Date : 2020-02-20 , DOI: 10.1080/00365513.2020.1729401
Bei Xu 1 , Yamei Zhang 1 , Yan Chen 2 , Min Zeng 3 , Jiafu Feng 1 , Jie Tang 1 , Lin Yu 1
Affiliation  

Few studies were reported on trace elements' alterations in uremic patients undergoing long-term (>24 months) hemodialysis (HD), especially by using the whole blood as the biological fluid for the measuring purpose. Our objective was to develop an improved micro-sampling inductively coupled plasma-mass spectrometry (ICP-MS) method to determine the levels of Ca, Mg, Cu, Zn, Fe, Mn, Se and Pb in uremic patients receiving long-term HD. A ICP-MS method with a modified whole blood sample preparation procedure with small volumes was established and applied for the simultaneous quantification of the various elements in uremic patients undergoing long-term HD. 124 eligible uremic patients receiving long-term HD (75 males and 49 females) and 77 healthy subjects (54 males and 23 females) were recruited and Ca, Mg, Cu, Zn, Fe, Mn, Se and Pb levels were further determined. Our results revealed that uremic patients with HD had significantly higher blood levels of Ca, Mg, Zn and Pb and lower Cu, Fe, Se and Mn concentrations than healthy controls. In conclusion, a reproducible and reliable ICP-MS method using minimal whole blood sample volume (50 μL) with a simple dilution-based preparation procedure was successfully improved, validated and applied. Uremic patients undergoing long-term HD might be at increased risk of some essential trace elements deficiency (especially for Cu, Fe and Se) or toxic trace element excess (Pb) in respect to healthy subjects. Monitoring of blood levels and supplementation of some trace elements may be indicated in uremic patients undergoing long-term HD.

中文翻译:

ICP-MS同时进行简单的全血样品稀释同时进行多元素分析,并将其应用于接受长期血液透析的尿毒症患者。

很少有研究报道了接受长期(> 24个月)血液透析(HD)的尿毒症患者微量元素的变化,特别是通过使用全血作为生物体液进行测量。我们的目标是开发一种改进的微采样电感耦合等离子体质谱法(ICP-MS),以确定接受长期HD治疗的尿毒症患者中Ca,Mg,Cu,Zn,Fe,Mn,Se和Pb的含量。建立了ICP-MS方法,该方法具有经过修改的小体积全血样品制备程序,并用于同时量化接受长期HD治疗的尿毒症患者的各种元素。招募了124例接受长期HD治疗的合格尿毒症患者(男性75例,女性49例)和77例健康受试者(54例男性和23例女性),并采集了Ca,Mg,Cu,Zn,Fe,Mn,硒和铅的水平进一步确定。我们的结果显示,与健康对照组相比,患有尿毒症的尿毒症患者血液中的钙,镁,锌和铅水平显着较高,而铜,铁,硒和锰的浓度较低。总之,成功地改进,验证和应用了使用最小的全血样品量(50μL)和基于稀释的简单制备方法的可重现和可靠的ICP-MS方法。进行长期HD的尿毒症患者相对于健康受试者而言,可能存在某些必需微量元素缺乏症(尤其是Cu,Fe和Se)或毒性微量元素过量(Pb)的风险增加。接受长期HD治疗的尿毒症患者可能需要监测血液水平并补充一些微量元素。我们的结果显示,与健康对照组相比,患有尿毒症的尿毒症患者血液中的钙,镁,锌和铅水平显着较高,而铜,铁,硒和锰的浓度较低。总之,成功地改进,验证和应用了使用最小的全血样品量(50μL)和简单的基于稀释的制备程序的可再现且可靠的ICP-MS方法。进行长期HD的尿毒症患者相对于健康受试者而言,可能存在某些必需微量元素缺乏症(尤其是Cu,Fe和Se)或毒性微量元素过量(Pb)的风险增加。接受长期HD治疗的尿毒症患者可能需要监测血液水平并补充一些微量元素。我们的结果显示,与健康对照组相比,患有尿毒症的尿毒症患者血液中的钙,镁,锌和铅水平显着较高,而铜,铁,硒和锰的浓度较低。总之,成功地改进,验证和应用了使用最小的全血样品量(50μL)和基于稀释的简单制备方法的可重现和可靠的ICP-MS方法。进行长期HD的尿毒症患者相对于健康受试者而言,可能存在某些必需微量元素缺乏症(尤其是Cu,Fe和Se)或毒性微量元素过量(Pb)的风险增加。接受长期HD治疗的尿毒症患者可能需要监测血液水平并补充一些微量元素。成功地改进,验证和应用了使用最小的全血样品量(50μL)和基于稀释的简单制备方法的可重复,可靠的ICP-MS方法。进行长期HD的尿毒症患者相对于健康受试者而言,可能存在某些必需微量元素缺乏症(尤其是Cu,Fe和Se)或毒性微量元素过量(Pb)的风险增加。接受长期HD治疗的尿毒症患者可能需要监测血液水平并补充一些微量元素。成功地改进,验证和应用了使用最小的全血样品量(50μL)和基于稀释的简单制备方法的可重复,可靠的ICP-MS方法。进行长期HD的尿毒症患者相对于健康受试者而言,可能存在某些必需微量元素缺乏症(尤其是Cu,Fe和Se)或毒性微量元素过量(Pb)的风险增加。接受长期HD治疗的尿毒症患者可能需要监测血液水平并补充一些微量元素。(Fe和Se)或有关健康受试者的有毒微量元素(Pb)。接受长期HD治疗的尿毒症患者可能需要监测血液水平并补充一些微量元素。(Fe和Se)或有关健康受试者的有毒微量元素(Pb)。接受长期HD治疗的尿毒症患者可能需要监测血液水平并补充一些微量元素。
更新日期:2020-04-18
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