Abstract

OSMAC approach was performed on the soft coral-derived fungus Trichoderma harzianum (XS-20090075) leading to the significant changes of its secondary metabolites by using two different cultures. A new naphthalene derivative, trichoharzin B (1) and a new natural product, methyl-trichoharzin (2) were isolated by using rice medium. Whereas, a new natural product, ethyl 2-bromo-4-chloroquinoline-3-carboxylate (9) was obtained by using Czapek’s medium. Their structures were established by extensive spectroscopic investigation. The absolute configuration of 5 was determined by single-crystal X-ray diffraction. Compound 9 was the first halogenate quinoline derivative isolated from the genus of Trichoderma.

摘要

OSMAC方法在软珊瑚衍生真菌哈茨木霉（XS-20090075）上进行，通过使用两种不同的培养物，其次生代谢产物发生了显着变化。使用稻米培养基分离出一种新的萘衍生物，滴虫蛋白B（1）和一种新的天然产物，甲基-trichoharzin（2）。而使用Czapek培养基获得了一种新的天然产物2-溴-4-氯喹啉-3-羧酸乙酯（9）。通过广泛的光谱研究确定了它们的结构。通过单晶X射线衍射确定5的绝对构型。化合物9是从木霉属中分离出的第一个卤代喹啉衍生物。