Hyaluronic Acid (HA)-Based Silk Fibroin/Zinc Oxide Core-Shell Electrospun Dressing for Burn Wound Management.,Macromolecular Bioscience

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Hyaluronic Acid (HA)-Based Silk Fibroin/Zinc Oxide Core-Shell Electrospun Dressing for Burn Wound Management.
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-02-19 , DOI: 10.1002/mabi.201900328
Zhina Hadisi _{1,

2} , Mehdi Farokhi ₃ , Hamid Reza Bakhsheshi-Rad ₄ , Maryam Jahanshahi _{1,

2} , Sadegh Hasanpour _{1,

2} , Erik Pagan _{1,

2} , Alireza Dolatshahi-Pirouz _{5,

6} , Yu Shrike Zhang ₇ , Subhas C Kundu ₈ , Mohsen Akbari _{1,

2}

Affiliation

Laboratory for Innovations in Microengineering (LiME), Department of Mechanical Engineering, University of Victoria, Victoria, BC, V8P 5C2, Canada.
Center for Advanced Materials and Related Technologies (CAMTEC), University of Victoria, Victoria, BC, V8P 5C2, Canada.
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, PO Box 1316943551, Iran.
Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Radboud university medical center, Radboud Institute for Molecular Life Sciences, Department of Dentistry-Regenerative Biomaterials, Philips van Leydenlaan 25, 6525EX, Nijmegen, The Netherlands.
Department of Health Technology, Institute of Biotherapeutic Engineering and Drug Targeting, Center for Intestinal Absorption and Transport of Biopharmaceuticals, Technical University of Denmark, Kgs Lyngby, 2800, Denmark.
Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne St, Cambridge, MA, 02139, USA.
3Bs Research Group, I3Bs-Institute on Biomaterials, biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, AvePark, Guimaraes, 4805-017, Portugal.

Burn injuries represent a major life‐threatening event that impacts the quality of life of patients, and places enormous demands on the global healthcare systems. This study introduces the fabrication and characterization of a novel wound dressing made of core–shell hyaluronic acid–silk fibroin/zinc oxide (ZO) nanofibers for treatment of burn injuries. The core–shell configuration enables loading ZO—an antibacterial agent—in the core of nanofibers, which in return improves the sustained release of the drug and maintains its bioactivity. Successful formation of core–shell nanofibers and loading of zinc oxide are confirmed by transmission electron microscopy, Fourier‐transform infrared spectroscopy, and energy dispersive X‐ray. The antibacterial activity of the dressings are examined against Escherichia coli and Staphylococcus aureus and it is shown that addition of ZO improves the antibacterial property of the dressing in a dose‐dependent fashion. However, in vitro cytotoxicity studies show that high concentration of ZO (>3 wt%) is toxic to the cells. In vivo studies indicate that the wound dressings loaded with ZO (3 wt%) substantially improves the wound healing procedure and significantly reduces the inflammatory response at the wound site. Overall, the dressing introduced herein holds great promise for the management of burn injuries.

中文翻译：

基于透明质酸（HA）的丝素蛋白/氧化锌核壳电纺敷料，用于烧伤创面管理。

烧伤是严重的威胁生命的事件，它影响患者的生活质量，并且对全球医疗系统提出了巨大的要求。这项研究介绍了由核-壳透明质酸-丝素蛋白/氧化锌（ZO）纳米纤维制成的新型伤口敷料的制备和表征，用于治疗烧伤。核-壳结构可以将ZO（一种抗菌剂）装载到纳米纤维的核中，从而改善了药物的持续释放并保持了其生物活性。透射电镜，傅立叶变换红外光谱和能量色散X射线证实了核-壳纳米纤维的成功形成和氧化锌的负载。检查敷料对大肠杆菌的抗菌活性和金黄色葡萄球菌和它表明除了ZO的提高以剂量依赖的方式将敷料的抗菌性。但是，体外细胞毒性研究表明，高浓度的ZO（> 3 wt％）对细胞有毒性。体内研究表明，载有ZO（3 wt％）的伤口敷料可显着改善伤口愈合过程，并显着降低伤口部位的炎症反应。总体而言，本文介绍的敷料对烧伤的处理具有广阔的前景。

更新日期：2020-02-19

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