"Clickable" and Antifouling Block Copolymer Brushes as a Versatile Platform for Peptide-Specific Cell Attachment.,Macromolecular Bioscience

当前位置： X-MOL 学术 › Macromol. Biosci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

"Clickable" and Antifouling Block Copolymer Brushes as a Versatile Platform for Peptide-Specific Cell Attachment.
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-02-19 , DOI: 10.1002/mabi.201900354
Rafał Poręba ₁ , Andres de Los Santos Pereira ₁ , Robert Pola ₁ , Siyu Jiang ₂ , Ognen Pop-Georgievski ₁ , Zdeňka Sedláková ₁ , Holger Schönherr ₂

Affiliation

To tailor cell–surface interactions, precise and controlled attachment of cell‐adhesive motifs is required, while any background non‐specific cell and protein adhesion has to be blocked effectively. Herein, a versatile and highly reproducible antifouling surface modification based on “clickable” groups and hierarchically structured diblock copolymer brushes for the controlled attachment of cells is reported. The polymer brush architecture combines an antifouling bottom block of poly(2‐hydroxyethyl methacrylate) poly(HEMA) and an ultrathin azide‐bearing top block, which can participate in well‐established “click” reactions including the highly selective copper‐catalyzed alkyne‐azide cycloaddition (CuAAC) reaction under mild conditions. This straightforward approach allows the rapid conjugation of a cell‐adhesive, alkyne‐bearing cyclic RGD peptide motif, enabling subsequent specific attachment of NIH 3T3 fibroblasts, their extensive proliferation and confluent cell sheet formation after 48 h of incubation. The generally applicable strategy presented in this report can be employed for surface functionalization with diverse alkyne‐bearing biological moieties via CuAAC or copper‐free alkyne‐azide cycloaddition protocols, making it a versatile functionalization approach and a promising tool for tissue engineering, biomaterial implant design, and other applications that require surfaces supporting highly specific cell attachment.

中文翻译：

“可点击的”和防污嵌段共聚物刷作为多肽特异性细胞附着的多功能平台。

为了适应细胞表面相互作用，需要精确且受控地粘附细胞粘附基序，同时必须有效阻止任何背景非特异性细胞和蛋白质粘附。在此，报告了一种基于“可点击”基团和分层结构的二嵌段共聚物刷的通用且可重复生产的防污表面改性剂，用于控制细胞的附着。聚合物刷结构将聚（2-甲基丙烯酸羟乙酯）聚（HEMA）的防污底块和带有超薄叠氮化物的顶块结合在一起，可以参与公认的“喀哒”反应，包括高度选择性的铜催化炔烃。在温和条件下进行叠氮化物环加成（CuAAC）反应。这种简单的方法可以快速结合细胞粘附剂，带有炔烃的环状RGD肽基序，可在培养48小时后实现NIH 3T3成纤维细胞的后续特异性附着，其广泛增殖和融合细胞片形成。本报告中介绍的普遍适用的策略可通过CuAAC或无铜炔叠氮化物环加成方案用于具有多种含炔生物部分的表面功能化，使其成为一种通用的功能化方法，并且是组织工程，生物材料植入物设计的有前途的工具以及需要表面支持高度特定的细胞附着的其他应用程序。

更新日期：2020-02-19

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11