Effects of Agrobacterium tumefaciens strain types on the Agrobacterium- mediated transformation efficiency of filamentous fungus Mortierella alpine,Letters in Applied Microbiology

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Effects of Agrobacterium tumefaciens strain types on the Agrobacterium- mediated transformation efficiency of filamentous fungus Mortierella alpine
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-05-01 , DOI: 10.1111/lam.13286
S Wang _{1,

2} , H Chen _{1,

2} , Y Wang _{1,

2} , C Pan _{1,

2} , X Tang _{1,

2} , H Zhang _{1,

2,

3,

4} , W Chen _{1,

2,

3,

5} , Y Q Chen _{1,

2,

3,

4,

6}

Affiliation

Four Agrobacterium tumefaciens strains AGL‐1, C58C1, EHA105 and LBA4404 were tested for the effects of strain types on the transformation efficiency in Mortierella alpina. Results showed that AGL‐1, C58C1 and EHA105 transformed M. alpina successfully. Among them, A. tumefaciens EHA105 was first proven successful transformation of M. alpina. AGL‐1 and EHA105 had the highest transformation efficiency among the four strains, while LBA4404 failed to transform M. alpina. The reason leading to the transformation efficiency difference among the four strains was explored by determining transcription levels of the virulence (vir) gene in the induction medium. Results showed that the expressions of virD1, virD2, virD4 and virE1 genes were obviously induced by acetosyringone in all the strains, and their transcriptional levels as well as virA’s of AGL‐1, C58C1 and EHA105 were higher than that of LBA4404, suggesting high transcriptional levels of vir genes were important for successful transformation. The study selected A. tumefaciens with high transformation efficiency of M. alpina, and would accelerate the genetic management of M. alpina.

中文翻译：

根癌农杆菌菌株类型对农杆菌介导的丝状真菌高山被孢霉转化效率的影响

测试了四种根癌农杆菌 AGL-1、C58C1、EHA105 和 LBA4404 菌株类型对高山被孢霉转化效率的影响。结果表明，AGL-1、C58C1和EHA105成功转化了高山木霉。其中，A. tumefaciens EHA105 首次被证明成功转化了高山木霉。AGL-1 和 EHA105 在 4 个菌株中转化效率最高，而 LBA4404 未能转化 M. alpina。通过测定诱导培养基中毒力（vir）基因的转录水平，探讨了导致四种菌株转化效率差异的原因。结果表明，所有菌株中virD1、virD2、virD4和virE1基因的表达均明显受乙酰丁香酮的诱导，其转录水平及AGL-1的virA，C58C1 和 EHA105 高于 LBA4404，表明 vir 基因的高转录水平对于成功转化很重要。该研究选择了高山木霉转化效率高的根癌农杆菌，将加速高山木霉的遗传管理。

更新日期：2020-05-01

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