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Pet dogs potential transmitters of pathogenic Escherichia coli with resistance to antimicrobials
Archives of Microbiology ( IF 2.3 ) Pub Date : 2020-02-19 , DOI: 10.1007/s00203-020-01828-9
X D Vega-Manriquez 1 , A Ubiarco-López 2, 3 , A Verdugo-Rodríguez 2 , U Hernández-Chiñas 3 , A Navarro-Ocaña 4 , R E Ahumada-Cota 3, 5 , D Ramírez-Badillo 1 , N Hernández-Díaz de León 1 , C A Eslava 3
Affiliation  

Escherichia coli strains are part of the normal biota of humans and animals; however, several clinical reports have implicated E. coli as the etiological agent of diarrhea in humans and companion animals. Thus, the aim of the present study was to know if companion dogs in the city of San Luis Potosi are colonized with virulent potentially harmful E. coli strains. Rectal swabs from 30 dogs, 13 with and 17 without diarrhea were analyzed. Phylogenetic and virulence genes analysis was performed to the E. coli isolates. Additionally, the Kirby-Bauer test was used to analyze the sensitivity to 32 different antimicrobials from 14 families. Eighty-five isolates were identified as E. coli and detected in 97% of healthy and diarrheic dog samples. E. coli isolates from healthy dogs carried several virulence genes, in contrast with those from diarrheic animals that presented only eaeA. In healthy dogs, phylogenetic analysis showed that 57% and 43% of E. coli isolates belonged to commensal (A and B1) and virulent (B2 and D) groups respectively. Meanwhile, diarrheic dogs showed that 69% of the isolates were identified as virulent B2 and D phylogroups. Moreover, E. coli resistant to β-lactams, aminoglycosides, tetracycline, quinolones, and folate inhibitors were detected in both groups of dogs. The presence of E. coli with eaeA virulence gene in diarrheic dogs, suggest that these strains are associated with the animal´s condition. Finally, major attention must be drawn to the careful handling of dogs because of their capability to harbor and disseminate virulent E. coli strains.

中文翻译:

宠物狗具有抗微生物药物耐药性的病原性大肠杆菌的潜在传播者

大肠杆菌菌株是人类和动物正常生物群的一部分;然而,一些临床报告表明大肠杆菌是人类和伴侣动物腹泻的病原体。因此,本研究的目的是了解圣路易斯波托西市的伴侣犬是否定植了有毒的潜在有害大肠杆菌菌株。分析了 30 只狗的直肠拭子,其中 13 只有腹泻,17 只没有腹泻。对大肠杆菌分离株进行了系统发育和毒力基因分析。此外,Kirby-Bauer 测试用于分析对来自 14 个家族的 32 种不同抗菌剂的敏感性。85 个分离株被鉴定为大肠杆菌,并在 97% 的健康和腹泻犬样本中检测到。从健康狗身上分离的大肠杆菌携带了几个毒力基因,与那些仅呈现 eaeA 的腹泻动物相反。在健康犬中,系统发育分析表明,57% 和 43% 的大肠杆菌分离株分别属于共生(A 和 B1)和毒性(B2 和 D)组。同时,腹泻犬显示 69% 的分离株被鉴定为毒力 B2 和 D 系统群。此外,在两组犬中均检测到对β-内酰胺类、氨基糖苷类、四环素、喹诺酮类和叶酸抑制剂耐药的大肠杆菌。腹泻犬中存在带有 eaeA 毒力基因的大肠杆菌,表明这些菌株与动物的病情有关。最后,必须特别注意对狗的小心处理,因为它们能够藏匿和传播有毒的大肠杆菌菌株。系统发育分析表明,57% 和 43% 的大肠杆菌分离株分别属于共生(A 和 B1)和毒性(B2 和 D)组。同时,腹泻犬显示 69% 的分离株被鉴定为毒力 B2 和 D 系统群。此外,在两组犬中均检测到对β-内酰胺类、氨基糖苷类、四环素、喹诺酮类和叶酸抑制剂耐药的大肠杆菌。腹泻犬中存在带有 eaeA 毒力基因的大肠杆菌,表明这些菌株与动物的病情有关。最后,必须特别注意对狗的小心处理,因为它们能够藏匿和传播有毒的大肠杆菌菌株。系统发育分析表明,57% 和 43% 的大肠杆菌分离株分别属于共生(A 和 B1)和毒性(B2 和 D)组。同时,腹泻犬显示 69% 的分离株被鉴定为毒力 B2 和 D 系统群。此外,在两组犬中均检测到对β-内酰胺类、氨基糖苷类、四环素、喹诺酮类和叶酸抑制剂耐药的大肠杆菌。腹泻犬中存在带有 eaeA 毒力基因的大肠杆菌,表明这些菌株与动物的病情有关。最后,必须特别注意对狗的小心处理,因为它们能够藏匿和传播有毒的大肠杆菌菌株。腹泻犬显示 69% 的分离株被鉴定为毒力 B2 和 D 系统群。此外,在两组犬中均检测到对β-内酰胺类、氨基糖苷类、四环素、喹诺酮类和叶酸抑制剂耐药的大肠杆菌。腹泻犬中存在带有 eaeA 毒力基因的大肠杆菌,表明这些菌株与动物的病情有关。最后,必须特别注意对狗的小心处理,因为它们能够藏匿和传播有毒的大肠杆菌菌株。腹泻犬显示 69% 的分离株被鉴定为毒力 B2 和 D 系统群。此外,在两组犬中均检测到对β-内酰胺类、氨基糖苷类、四环素、喹诺酮类和叶酸抑制剂耐药的大肠杆菌。腹泻犬中存在带有 eaeA 毒力基因的大肠杆菌,表明这些菌株与动物的病情有关。最后,必须特别注意对狗的小心处理,因为它们能够藏匿和传播有毒的大肠杆菌菌株。
更新日期:2020-02-19
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