Transient receptor potential vanilloid 1 (TRPV1) is a polymodal receptor channel, which plays an important role in pain transduction. It is important to understand the functional expression of this channel under neuropathic pain (NP) conditions. A novel method was used to investigate the dynamics of functional expression of this channel on regenerated neurons under NP conditions following trigeminal nerve injury using a combination of a permanently charged sodium channel blocker (QX-314) and a TRPV1 agonist (capsaicin; QX-CAP). The combination was originally introduced as a local anesthetic. Synchronization between the local anesthetic effect of QX-CAP and TRPV1 expression on regenerated neurons was observed following the nerve injury. QX-CAP had no local anesthetic effect under NP conditions 2 weeks after the injury when TRPV1 expression on regenerated neurons was low. However, this combination was effective under NP conditions 3 and 4 weeks following injury when TRPV1 expression in regenerated neurons was moderate to high. The current review, discusses the potential of QX-314 as a local anesthetic and a novel approach of using QX-CAP to reveal the dynamics of functional expression of TRPV1 on regenerated neurons following trigeminal nerve injury.

中文翻译：

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检测神经损伤后再生神经元上TRPV1通道功能表达的新方法。

瞬态受体电位香草酸1（TRPV1）是一种多峰受体通道，在疼痛传导中起重要作用。重要的是要了解该通道在神经性疼痛（NP）条件下的功能表达。一种新方法用于研究三叉神经损伤后NP条件下该通道在NP条件下再生神经元上的功能性表达的动力学，方法是使用永久充电的钠通道阻滞剂（QX-314）和TRPV1激动剂（辣椒素； QX-CAP）联合使用）。该组合最初是作为局部麻醉剂引入的。神经损伤后，观察到QX-CAP的局部麻醉作用与TRPV1表达对再生神经元的同步性。损伤后2周，当再生神经元上TRPV1表达低时，QX-CAP在NP条件下无局部麻醉作用。但是，当再生神经元中TRPV1表达为中到高水平时，这种组合在NP损伤后3和4周有效。当前的评论讨论了QX-314作为局麻药的潜力以及使用QX-CAP揭示三叉神经损伤后再生神经元上TRPV1功能表达动态的新方法。