Coenzyme Q₁₀ (CoQ₁₀) is a strongly hydrophobic lipid that functions in the electron transport chain and as an antioxidant. CoQ₁₀ was conferred with aqueous solubility by incorporation into nanoparticles containing phosphatidylcholine (PtdCho) and apolipoprotein (apo) A‐I. These particles, termed CoQ₁₀ nanodisks (ND), contain 1.0 mg CoQ₁₀/5 mg PtdCho/2 mg apoA‐I (97% CoQ₁₀ solubilization efficiency). UV/Vis absorbance spectroscopy of CoQ₁₀ ND revealed a characteristic absorbance peak centered at 275 nm. Incorporation of CoQ₁₀ into ND resulted in quenching of apoA‐I tryptophan fluorescence emission. Gel filtration chromatography of CoQ₁₀ ND gave rise to a single major absorbance peak and HPLC of material extracted from this peak confirmed the presence of CoQ₁₀. Incubation of cultured cells with CoQ₁₀ ND, but not empty ND, resulted in a significant increase in the CoQ₁₀ content of mitochondria as well as enhanced oxidative phosphorylation, as observed by a ~24% increase in maximal oxygen consumption rate. Collectively, a facile method to solubilize significant quantities of CoQ₁₀ in lipid nanoparticles has been developed. The availability of CoQ₁₀ ND provides a novel means to investigate biochemical aspects of CoQ₁₀ uptake by cells and/or administer it to subjects deficient in this key lipid as a result of inborn errors of metabolism, statin therapy, or otherwise.

中文翻译：

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富含生物相容性辅酶Q10的脂质纳米颗粒的组装和表征。

辅酶Q ₁₀（CoQ ₁₀）是一种强疏水性脂质，在电子传输链中起作用并作为抗氧化剂。通过将CoQ ₁₀掺入含有磷脂酰胆碱（PtdCho）和载脂蛋白（apo）A-1的纳米颗粒中，使其具有水溶性。这些颗粒，称为辅酶Q _10个纳米盘（ND），含有1.0毫克的CoQ ₁₀ / 5mg的磷脂酰胆碱/ 2毫克的apoA-I（97％的CoQ ₁₀溶解效率）。CoQ ₁₀ ND的UV / Vis吸收光谱显示了一个集中在275 nm处的特征吸收峰。合并辅酶Q ₁₀进入ND会导致apoA-I色氨酸荧光发射猝灭。辅酶Q ₁₀ ND的凝胶过滤色谱法产生一个主要的吸光度峰，从该峰中提取的材料的HPLC证实了辅酶Q ₁₀的存在。用CoQ ₁₀ ND（而非空ND）孵育培养的细胞后，线粒体的CoQ ₁₀含量显着增加，并且氧化磷酸化增强，最大耗氧率增加了约24％。集体地，已经开发了一种在脂质纳米颗粒中溶解大量CoQ ₁₀的简便方法。CoQ ₁₀的可用性ND提供了一种新颖的方法来研究细胞摄取辅酶Q _10的生化方面和/或将其施用给由于先天性代谢异常，他汀类药物治疗或其他原因而缺乏该关键脂质的受试者。