Several plant pathogens severely affect crop yield and quality, thereby threatening global food security. In order to cope with this challenge, genetic improvement of plant disease resistance is required for sustainable agricultural production, for which conventional breeding is unlikely to do enough. Luckily, genome editing systems that particularly clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) has revolutionized crop improvement by enabling robust and precise targeted genome modifications. It paves the way towards new methods for genetic improvement of plant disease resistance and accelerates resistance breeding. In this review, the challenges, limitations and prospects for conventional breeding and the applications of CRISPR/Cas9 system for the development of transgene-free disease-resistant crops are discussed.

中文翻译：

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用于植物抗病性发展的CRISPR / Cas9：最新进展，局限性和未来前景。

几种植物病原体严重影响农作物的产量和质量，从而威胁全球粮食安全。为了应对这一挑战，可持续农业生产需要提高植物抗病性的基因，而常规育种不可能做到这一点。幸运的是，特别是将规则间隔的短回文重复序列（CRISPR）/ CRISPR相关蛋白9（CRISPR / Cas9）聚在一起的基因组编辑系统通过实现强大而精确的靶向基因组修饰，彻底改变了作物改良方法。它为植物抗病性遗传改良的新方法铺平了道路，并加速了抗性育种。在这篇评论中，挑战，