ABSTRACT

Transmission of Pseudomonas aeruginosa along the food chain could cause gastrointestinal infections. To show this involvement, the prevalence, putative virulence genotype, and antibiotic resistance phenotype of P. aeruginosa isolates from stool of 1482 patients with community and hospital acquired diarrhea were compared with 87 isolates from the environmental samples. The results showed infection with P. aeruginosa in 3.4% of the cases, while 57.4% of vegetable samples were contaminated. Significantly higher frequency of lasB (98%), aprA (98%), exoY (98%), and exoS (90%), but lower rate of exoT (39.2%), was detected among the stool isolates. Multi-drug resistance (MDR) phenotype was detected in 25.5% and 4% of the stool and vegetable isolates, respectively. A higher rate of studied virulence genes was detected among the MDR strains vs non-MDR strains. These results indicate P. aeruginosa as a causative agent of diarrhea either among the hospitalized patients and those with community-acquired diarrhea.

摘要

铜绿假单胞菌沿食物链传播可能导致胃肠道感染。为了证明这种参与，将 1482 名社区和医院获得性腹泻患者粪便中的铜绿假单胞菌分离株与环境样本中的 87 株分离株的流行率、假定的毒力基因型和抗生素耐药表型进行了比较。结果显示，3.4%的病例感染了铜绿假单胞菌，而57.4%的蔬菜样本被污染。lasB (98%)、aprA (98%)、exoY (98%) 和exoS (90%)的频率显着增加，但exoT的发生率较低(39.2%)，在粪便分离物中检测到。分别在 25.5% 和 4% 的粪便和蔬菜分离物中检测到多药耐药 (MDR) 表型。在 MDR 菌株与非 MDR 菌株之间检测到更高的研究毒力基因率。这些结果表明，在住院患者和社区获得性腹泻患者中，铜绿假单胞菌是腹泻的病原体。