To evaluate the effects of whey protein (WP) sources on performance, liver antioxidants and immune responses of broiler chickens, 300 one-d-old male Ross 308 chickens were randomly allocated into six treatments with five replications of 10 birds each. This study was conducted on the basis of a completely randomized design in a 3 × 2 factorial arrangement with dietary supplemental WP sources (no WP; 2% WP and 2% whey protein concentrate (WPC)) and ethanol (zero and 6%) into drinking water. Birds were challenged with ethanol 6% from 10 to 24 days of age. Growth performance, immune responses, intestinal morphology and antioxidant enzymes were assessed in both challenged and non-challenged groups. There was a significant interaction between ethanol and WP sources for growth performance, so that weight gain (WG), feed intake (FI) and feed conversion ratio (FCR) were significantly improved in broiler chickens challenged with ethanol and fed with WP (p < .05). Intestinal morphology, humoral responses and heterophil to lymphocyte ratio (H/L) were not affected by WP sources. Cellular immunity responses were significantly lower in control group and challenged birds fed with WP compared with other groups after 48 hr of phytohemagglutinin-P (PHA-P) injection. Ethanol challenge decreased liver antioxidant enzyme activities and increased levels of malondialdehyde (MDA) and alanine aminotransferase (ALT) activities. Dietary inclusion of WP and WPC increased levels of liver antioxidant enzymes and decreased MDA and ALT activities. It can be recommended to use WP for improving the growth performance and liver antioxidant enzymes in broiler chickens challenged with ethanol.

中文翻译：

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乳清蛋白来源对用乙醇挑战的肉鸡的性能，肝脏抗氧化剂和免疫反应的评估。

为了评估乳清蛋白（WP）来源对肉鸡生产性能，肝脏抗氧化剂和免疫反应的影响，将300只一岁雄性Ross 308鸡随机分配到六种处理中，每组10只，重复五次。这项研究是在3×2因数排列的完全随机设计的基础上进行的，其中膳食补充WP来源（无WP； 2％WP和2％乳清蛋白浓缩物（WPC））和乙醇（零和6％）进入饮用水。从10到24日龄，用6％的乙醇攻击鸟类。在挑战组和非挑战组中均评估了生长性能，免疫反应，肠道形态和抗氧化酶。乙醇和可湿性粉剂来源之间存在显着的相互作用，以提高生长性能，因此体重增加（WG），在用乙醇挑战和饲喂WP的肉鸡中，采食量（FI）和饲料转化率（FCR）显着提高（p <.05）。肠道形态，体液反应和嗜异性淋巴细胞比（H / L）不受WP来源的影响。注射植物血凝素-P（PHA-P）48小时后，对照组和用WP喂养的受攻击禽类的细胞免疫应答明显低于其他组。乙醇挑战降低了肝脏抗氧化酶的活性，并增加了丙二醛（MDA）和丙氨酸氨基转移酶（ALT）的水平。膳食中WP和WPC的摄入增加了肝脏抗氧化酶的水平，降低了MDA和ALT活性。