The American eel (Anguilla rostrata) is an imperilled fish hypothesized to use conspecific cues, in part, to coordinate long distance migration during their multi-stage life history. Here, holding water and tissue from multiple American eel life stages was collected and analysed for the presence, profile, and concentration of bile acids. Distinct bile acid profiles were identified in glass, elver, yellow eel, and silver eel holding waters using ultra-performance liquid chromatography high-resolution mass spectrometry (UPLC-HRMS) and principal component analysis. Taurochenodeoxycholic acid, taurodeoxycholic acid, cholic acid, deoxycholic acid, taurolithocholic acid, and taurocholic acid were detected in whole tissue of American glass eels and elvers, and in liver, intestine, and gall bladder samples of late stage yellow eels. Bile acids were not a major component of silver eel washings or tissue. This study is novel because little was previously known about bile acids produced and emitted into the environment by American eels. Future behavioural studies could evaluate whether any bile acids produced by American eels influence conspecific migratory behaviour. This article is protected by copyright. All rights reserved.

中文翻译：

---

美国鳗鱼产生和释放胆汁酸谱，在不同的生命阶段有所不同

美洲鳗鱼 (Anguilla rostrata) 是一种濒危鱼类，假设使用同种线索，部分是为了在其多阶段生活史中协调长距离迁徙。在这里，收集了来自多个美国鳗鱼生命阶段的水分和组织，并分析了胆汁酸的存在、分布和浓度。使用超高效液相色谱高分辨率质谱 (UPLC-HRMS) 和主成分分析在玻璃、鳗鱼、黄鳗和银鳗保存水中鉴定出不同的胆汁酸谱。牛磺鹅去氧胆酸、牛磺脱氧胆酸、胆酸、脱氧胆酸、牛磺石胆酸和牛磺胆酸在美洲玻璃鳗和鳗的全组织以及晚期黄鳗的肝脏、肠道和胆囊样本中检测到。胆汁酸不是银鳗洗液或组织的主要成分。这项研究是新颖的，因为以前对美洲鳗产生和排放到环境中的胆汁酸知之甚少。未来的行为研究可以评估美洲鳗产生的任何胆汁酸是否会影响同种迁徙行为。本文受版权保护。版权所有。