Diploid wild einkorn wheat, Triticum boeoticum Boiss (A^bA^b, 2n = 2x = 14), is a wheat-related species with a blue aleurone layer. In this study, six blue-grained wheat lines were developed from F₈ progeny of crosses between common wheat and T. boeoticum. The chromosome constitutions of these lines were characterized by fluorescence in situ hybridization (FISH) using the oligonucleotide probes Oligo-pTa535-1, Oligo-pSc119.2-1, Oligo-pTa71-2, and (AAC)₇. Multicolor FISH using Oligo-pTa535-1, Oligo-pSc119.2-1, and Oligo-pTa71-2 identified all 42 common wheat chromosomes, while Oligo-pTa535-1 and (AAC)₇ discriminated the 14 chromosomes of T. boeoticum. FISH revealed that all six blue-grained lines were wheat-T. boeoticum 4A^b (4B) disomic substitution lines. The substitution lines were validated by genotyping using the wheat 55 K single nucleotide polymorphism (SNP) array containing 53,063 markers. These 4A^b (4B) substitution lines represent novel germplasm for blue-grained wheat breeding. The FISH probes and SNP markers used here can be applied in the development of blue-grained wheat-Triticum boeoticum translocation lines.

中文翻译：

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蓝粒小麦小麦小麦替代品系的开发，鉴定和表征。

二倍体野生einkorn小麦，Triticum boeoticum Boiss（A ^b A ^b，2 n  = 2 x  = 14），是一种与小麦相关的种，具有蓝色糊粉层。在这项研究中，从普通小麦和T. boeoticum杂交的F ₈后代中培育了六种蓝粒小麦品系。使用寡核苷酸探针Oligo-pTa535-1，Oligo-pSc119.2-1，Oligo-pTa71-2和（AAC）₇通过荧光原位杂交（FISH）表征这些细胞系的染色体组成。使用Oligo-pTa535-1，Oligo-pSc119.2-1和Oligo-pTa71-2进行的多色FISH鉴定了全部42条普通小麦染色体，而Oligo-pTa535-1和（AAC）₇鉴别了T. boeoticum的14条染色体。FISH显示，所有六个蓝粒系均为小麦-棉铃虫4A ^b（4B）二体组替代系。通过使用包含53,063个标记的小麦55 K单核苷酸多态性（SNP）阵列通过基因分型来验证替代品系。这些4A ^b（4B）替代系代表了蓝粒小麦育种的新种质。此处使用的FISH探针和SNP标记可用于开发蓝粒小麦-小麦易位系。