91H, a long non-coding antisense transcripts located on the position of the H19/IGF2 locus had been suggested to play a critical role in tumour development. However, little study had proved the mechanism in colorectal cancer (CRC). Hence, we performed this study to deeply explore the mechanism of lncRNA 91H in tumour progression. The expression of lncRNA 91H was first detected in CRC tissues and cells which was higher in vitro and in vivo than normal cells or tissues and CRC patients with high lncRNA 91H expression usually had a high risk in tumour metastasis (p < .05). Then, monodansylcadaverine (MDC) staining, scratch wound, migration and invasion assays were conducted which showed to that reduced lncRNA 91H would greatly affect tumour migration, invasion and autophagy. Finally, by RNA pull down and RNA-binding protein immunoprecipitation (RIP) assay, a significant interaction was found between lncRNA 91H and IGF2BP2 which was proved to play an important role in CRC IGF2 expression. All these results suggested lncRNA 91H promotes IGF2 expression by interacting with IGF2BP2 which would provide a new strategy in finding potential CRC diagnostic biomarkers and therapeutic targets.

中文翻译：

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长链非编码 RNA 91H 通过与 IGF2BP2 相互作用调节 IGF2 表达并促进结直肠癌的肿瘤发生。

91H，一个位于 H19/IGF2 基因座位置的长非编码反义转录物被认为在肿瘤发展中起关键作用。然而，很少有研究证明结直肠癌（CRC）的机制。因此，我们进行了这项研究，以深入探讨 lncRNA 91H 在肿瘤进展中的机制。lncRNA 91H 的表达首先在 CRC 组织和细胞中检测到，其在体外和体内均高于正常细胞或组织，并且具有高 lncRNA 91H 表达的 CRC 患者通常具有较高的肿瘤转移风险（p < .05）。然后，进行单丹磺酰尸胺（MDC）染色、划伤、迁移和侵袭试验，结果表明减少的lncRNA 91H会极大地影响肿瘤的迁移、侵袭和自噬。最后，通过 RNA pull down 和 RNA 结合蛋白免疫沉淀 (RIP) 测定，发现 lncRNA 91H 和 IGF2BP2 之间存在显着的相互作用，这被证明在 CRC IGF2 表达中起重要作用。所有这些结果表明，lncRNA 91H 通过与 IGF2BP2 相互作用促进 IGF2 表达，这将为寻找潜在的 CRC 诊断生物标志物和治疗靶点提供新的策略。