Diabetes is a proinflammatory and prothrombotic condition that increases the risk of vascular complications. The aim of this study was to develop a diabetic microvascular flow model that allows to study the complex interactions between endothelial cells, blood cells and plasma proteins and their effects on clot formation. Primary human cardiac microvascular endothelial cells from donors without diabetes or donors with diabetes (type 1 or type 2) were grown in a microfluidic chip, perfused with non-diabetic or diabetic whole blood, and clot formation was assessed by measuring fibrin deposition in real time by confocal microscopy. Clot formation in non-diabetic whole blood was significantly increased in the presence of endothelial cells from donors with type 2 diabetes compared with cells from donors without diabetes. There was no significant difference in clot formation between non-diabetic and diabetic whole blood. We present for the first time a diabetic microvascular flow model as a new tool to study clot formation as a result of the complex interactions between endothelial cells, blood cells and plasma proteins in a diabetes setting. We show that endothelial cells affect clot formation in whole blood, attributing an important role to the endothelium in the development of atherothrombotic complications.

中文翻译：

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糖尿病影响微血管流动模型中的内皮细胞功能并改变纤维蛋白凝块形成：一项先导研究。

糖尿病是一种促炎性和血栓性疾病，会增加血管并发症的风险。这项研究的目的是建立一个糖尿病微血管流动模型，该模型可以研究内皮细胞，血细胞和血浆蛋白之间的复杂相互作用及其对血凝块形成的影响。将来自没有糖尿病的供体或患有糖尿病的供体（1型或2型）的原代人心脏微血管内皮细胞培养在微流控芯片中，并灌注非糖尿病或糖尿病全血，并通过实时测量血纤蛋白沉积评估血凝块形成通过共聚焦显微镜。与没有糖尿病的捐献者的细胞相比，在患有2型糖尿病的捐献者的内皮细胞存在的情况下，非糖尿病全血中的血凝块形成显着增加。非糖尿病和糖尿病全血之间的凝块形成没有显着差异。我们首次提出糖尿病微血管血流模型，作为研究由于糖尿病环境中内皮细胞，血细胞和血浆蛋白之间复杂相互作用而形成凝块的一种新工具。我们表明内皮细胞影响全血中的血块形成，在动脉粥样硬化血栓形成并发症的发展中归因于内皮的重要作用。