In the 1980s, I found that the chromosomal β-lactamase of Klebsiella pneumoniae LEN-1 showed a very high similarity to the R-plasmid-mediated penicillinase TEM-1 on amino acid sequence level, and this strongly suggested the TEM-1 originated from the chromosomal penicillinases of K. pneumoniae or related bacteria. Moreover, the chromosomal K1 β-lactamase (KOXY) of Klebsiella oxytoca was found to belong to the class A β-lactamases including LEN-1 and TEM-1, despite the KOXY can hydrolyze cefoperazone like the chromosomal AmpC-type cephalosporinases of various Enterobacteriaceae can hydrolyze several cephalosporins including cefoperazone. Furthermore, my collaborators and I also found plural novel serine-type β-lactamases such as MOX-1, SHV-24, TEM-91, CTX-M-64, CMY-9, CMY-19, GES-3, GES-4, and TLA-3, mediated by plasmids. Besides these serine-type β-lactamases, we also firstly identified exogenously acquired metallo-β-lactamases (MBL), IMP-1 and SMB-1, in imipenem resistant Serratia marcescens, and the IMP-1-producing S. marcescens TN9106 became the index case for carbapenemase-producing Enterobacteriaceae (CPE). I developed the SMA-disk test for the simple identification of the MBL-producing bacteria. We were also the first to identify a variety of plasmid-mediated 16S rRNA methyltransferases, RmtA, RmtB, RmtC, and NpmA, from various Gram-negative bacteria that showed very high levels of resistance to a wide range of aminoglycosides. Furthermore, we also firstly found plasmid-mediated quinolone efflux pump, QepA, and fosfomycin-inactivating enzymes, FosA3 and FosK. We also firstly characterized penicillin reduced susceptible Streptococcus agalactiae, macrolide-resistant Mycoplasma pneumoniae, Campylobacter jejuni, and Helicobacter pylori, together with carbapenem-resistant Haemophilus influenzae. We constructed a PCR-based ORF typing method for rapid identification of Acinetobacter baumannii international clones. This article is protected by copyright. All rights reserved.

中文翻译：

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克服新出现的耐多药细菌的系统研究。

在1980年代，我发现肺炎克雷伯氏菌LEN-1的染色体β-内酰胺酶在氨基酸序列水平上与R质粒介导的青霉素酶TEM-1具有非常高的相似性，这强烈表明TEM-1起源于肺炎克雷伯氏菌或相关细菌的染色体青霉菌。此外，尽管产氧杆菌能够水解头孢哌酮，如各种肠杆菌科细菌的染色体AmpC型头孢菌素酶一样，但发现氧化克雷伯氏菌的染色体K1β-内酰胺酶（KOXY）属于A类β-内酰胺酶，包括LEN-1和TEM-1。可以水解包括头孢哌酮在内的几种头孢菌素。此外，我和我的合作者还发现了多种新型的丝氨酸型β-内酰胺酶，例如MOX-1，SHV-24，TEM-91，CTX-M-64，CMY-9，CMY-19，GES-3，GES- 4，和TLA-3，由质粒介导。除这些丝氨酸型β-内酰胺酶外，我们还首先在耐亚胺培南的粘质沙雷氏菌中鉴定了外源性金属β-内酰胺酶（MBL），IMP-1和SMB-1，并且产生IMP-1的粘菌S. marcescens TN9106成为产生碳青霉烯酶的肠杆菌科（CPE）的索引案例。我开发了SMA磁盘测试，用于简单识别产生MBL的细菌。我们也是第一个从各种革兰氏阴性细菌中鉴定出多种质粒介导的16S rRNA甲基转移酶，RmtA，RmtB，RmtC和NpmA的人，这些细菌对多种氨基糖苷类表现出很高的抗性。此外，我们还首先发现了质粒介导的喹诺酮外排泵QepA和磷霉素灭活酶FosA3和FosK。我们还首先表征了青霉素降低的易感性无乳链球菌，耐大环内酯性肺炎支原体，空肠弯曲杆菌和幽门螺杆菌，以及耐碳青霉烯的流感嗜血杆菌。我们构建了基于PCR的ORF分型方法，用于快速鉴定鲍曼不动杆菌国际克隆。本文受版权保护。版权所有。