Aim: The encapsulation of Trichoderma asperellum BRM-29104 using Ca-alginate matrix was evaluated. Methods: Intact and freeze-dried beads containing submerged conidia and microsclerotia (MS) of T. asperellum grown in liquid culture were prepared. Beads were stored at 8, 25 and 35 °C over 120 days. Results: The mean sizes of beads before and after freeze-drying were 2.5 ± 0.2 mm and 1.5 × 1.1 mm (± 0.1 mm), respectively. Freeze-dried beads stored at 8 °C were more effective in maintaining conidia concentration, and MS concentrations were 102 MS/g for both beads at 8 and 25 °C. The concentration of viable cells in freeze-dried beads stored at 8 °C attained 3.0 × 108 CFU/g after 120 days. FIRT analysis showed an interaction between the alginate and the cell wall of the fungus. Conclusion: These findings support the use of alginate beads followed by freeze drying and cold storage for maintenance of viability of T. asperellum.

中文翻译：

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海藻酸盐珠粒中封装的曲霉木霉的制备，表征和细胞活力。

目的：评估了使用海藻酸钙基质对曲霉木霉BRM-29104的包封。方法：制备完整的冻干珠粒，该珠粒包含在液体培养中生长的米粉锥菌的淹没分生孢子和微菌核（MS）。将珠粒在8、25和35°C下保存120天。结果：冷冻干燥前后，珠粒的平均大小分别为2.5±0.2 mm和1.5×1.1 mm（±0.1 mm）。储存在8°C的冻干珠子在保持分生孢子浓度方面更有效，在8和25°C下，两种珠子的MS浓度均为102 MS / g。120天后，保存在8°C的冷冻干燥珠中的活细胞浓度达到3.0×108 CFU / g。FIRT分析显示藻酸盐和真菌细胞壁之间有相互作用。结论：