Despite its low virulence potential and a commensal lifestyle as a member of the human skin microbiota, Brevibacterium casei has been increasingly reported as an opportunistic pathogen, especially in immunocompromised patients. Here, we present the draft genome sequence of the S51 strain isolated from a bloodstream infection. To the best of the authors’ knowledge, this is the first report of the draft genome sequence of the B. casei strain isolated from the clinical infection. The strain was identified using phenotypic and molecular methods and subsequently sequenced using the next-generation sequencing. The draft whole genome was assembled de novo, automatically annotated by Rapid Annotations using Subsystems Technology (RAST) server and scrutinized to predict the presence of virulence, resistance, and stress response proteins. The genome size of the S51 strain was 3,743,532 bp and an average G+C content was 68.3%. The predicted genes included 48 genes involved in resistance to antibiotics (including vancomycin, fluoroquinolones, and beta-lactams) and toxic compounds (heavy metals), 16 genes involved in invasion and intracellular resistance (Mycobacterium virulence operons), and 94 genes involved in stress response (osmotic, oxidative stress, cold and heat shock). ResFinder has indicated the presence of a beta-lactamase, and a phenotypic analysis showed resistance to penicillin. This whole-genome NGS project for the S51strain has been deposited at EMBL/GenBank under the accession no. QNGF00000000. Electronic supplementary material The online version of this article (10.1007/s42770-020-00236-x) contains supplementary material, which is available to authorized users.

中文翻译：

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从血流感染中分离的干酪短杆菌菌株的全基因组序列草图

尽管其低毒力潜力和作为人类皮肤微生物群成员的共生生活方式，但越来越多的报道称干酪短杆菌是一种机会性病原体，尤其是在免疫功能低下的患者中。在这里，我们展示了从血流感染中分离出的 S51 菌株的基因组序列草图。据作者所知，这是从临床感染中分离出的干酪乳杆菌菌株基因组序列草图的第一份报告。该菌株使用表型和分子方法进行鉴定，随后使用下一代测序进行测序。全基因组草图从头组装，通过使用子系统技术 (RAST) 服务器的快速注释自动注释，并仔细检查以预测毒力、抗性和应激反应蛋白的存在。S51 菌株的基因组大小为 3,743,532 bp，平均 G+C 含量为 68.3%。预测基因包括48个涉及抗生素（包括万古霉素、氟喹诺酮类和β-内酰胺类）和有毒化合物（重金属）抗性的基因，16个涉及侵袭和细胞内抗性的基因（分枝杆菌毒力操纵子）和94个涉及应激的基因反应（渗透压、氧化应激、冷和热休克）。ResFinder 已表明存在 β-内酰胺酶，表型分析显示对青霉素有抗药性。该 S51 菌株的全基因组 NGS 项目已存放在 EMBL/GenBank，保藏号为。QNGF00000000。电子补充材料本文的在线版本（10.1007/s42770-020-00236-x）包含补充材料，可供授权用户使用。