The evolutionarily conserved multiprotein complex THO/TREX is required for pre-mRNA processing, mRNA export and the maintenance of genome stability. In this study, we analyzed the genome-wide distribution of human THOC7, a component of human THO, by chromatin immunoprecipitation sequencing. The analysis revealed that human THOC7 occupies repetitive sequences, which include microsatellite repeats in genic and intergenic regions and telomeric repeats. The majority of the THOC7 ChIP peaks overlapped with those of the elongating form of RNA polymerase II and R-loops, indicating that THOC7 accumulates in transcriptionally active repeat regions. Knocking down THOC5, an RNA-binding component of human THO, by siRNA induced the accumulation of γH2AX in the repeat regions. We also observed an aberration in the telomeres in the THOC5-depleted condition. These results suggest that human THO restrains the transcription-associated instability of repeat regions in the human genome.

中文翻译：

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人类THO维持重复DNA的稳定性。

进化上保守的多蛋白复合物THO / TREX是mRNA预处理，mRNA输出和基因组稳定性维持所必需的。在这项研究中，我们通过染色质免疫沉淀测序分析了人类THOC7（人类THO的一个组成部分）的全基因组分布。分析显示，人THOC7占据重复序列，其中包括基因和基因间区域的微卫星重复序列和端粒重复序列。大多数THOC7 ChIP峰与伸长形式的RNA聚合酶II和R环重叠，表明THOC7积累在转录活性重复区域中。通过siRNA敲除THOC5（一种人类THO的RNA结合成分）诱导了γH2AX在重复区域的积累。我们还观察到THOC5耗尽状态下端粒的畸变。