Cacay oil and butter were evaluated as enzymatic inducers for lipase production from Aspergillus terreus NRRL-255 by solid-state fermentation (SSF). Initially, physicochemical characteristics of agro-industrial wastes were evaluated in order to identify a potential solid substrate for lipase production. Higher water absorption index (3.65 g H₂O/g substrate), adequate mineral content, great carbon source, and nitrogen concentration were factors that influenced the choice of wheat bran as a solid substrate. Cacay butter presented the highest lipolytic activity (308.14 U g⁻¹) in the screening of lipid inducer. Then, the effects of lipid inducer concentration (cacay butter), temperature, pH, moisture, and fermentation time were evaluated on process performance using multivariate statistical methodology. Under optimal conditions, the highest lipase activity observed was 2,867.18 U g⁻¹. Regarding the lipase characterization, maximum relative activity was obtained at pH 7.0 and at 35 °C. An inhibitory effect was observed for Ca²⁺, Mn²⁺, Zn²⁺, Fe²⁺, and Cu²⁺ ions. Lipase activity was increased with the reduction of sodium dodecyl sulfate (SDS) concentration and the increase of Triton X-100. Therefore, the use of wheat bran as a solid substrate combined with cacay butter demonstrated a substantial lipase production, indicating its biotechnological industrial potential.

评价可可油和黄油是通过固态发酵（SSF）从土曲霉NRRL-255生产脂肪酶的酶促剂。最初，对农业工业废料的物理化学特性进行了评估，以确定潜在的脂肪酶生产固体底物。较高的吸水率（3.65 g H ₂ O / g底物），充足的矿物质含量，大量的碳源和氮浓度是影响小麦糠作为固体底物选择的因素。可可脂具有最高的脂解活性（308.14 U g ^-1）在脂质诱导剂的筛选中。然后，使用多元统计方法评估脂质诱导剂浓度（cacay butter），温度，pH，湿度和发酵时间对过程性能的影响。在最佳条件下，观察到的最高脂肪酶活性为2,867.18 U g ^-1。关于脂肪酶的表征，在pH 7.0和35°C下获得了最大的相对活性。观察到对Ca 2 ⁺，Mn 2 ⁺，Zn 2 ⁺，Fe ²⁺和Cu ²⁺有抑制作用。离子。脂肪酶活性随十二烷基硫酸钠（SDS）浓度的降低和Triton X-100的升高而增加。因此，使用麦麸作为固体基质与可可脂的组合物证明了脂肪酶的大量生产，表明了其生物技术的工业潜力。