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Discovery and molecular characterization of a novel trichovirus infecting sweet cherry.
Virus Genes ( IF 1.9 ) Pub Date : 2020-02-17 , DOI: 10.1007/s11262-020-01743-7
Eric Brewer 1 , Mengji Cao 1, 2 , Benjamin Gutierrez 3 , Margarita Bateman 4 , Ruhui Li 1
Affiliation  

Contigs with the highest sequence similarity (73%) to Apricot pseudo-chlorotic leaf spot virus (genus Trichovirus, family Betaflexiviridae) were identified by high-throughput sequencing from a symptomless sweet cherry accession. The complete genome sequence of this new virus is 7460 nucleotides, excluding the 3' poly(A) tail. Its genome organization is very similar to several trichoviruses infecting fruit trees, with three open reading frames encoding putative replicase, movement protein and coat protein (CP). The virus shares amino acid sequence identities of 60-73% at replicase and 53-76% at CP with other trichoviruses. Phylogenetic analyses group it and other trichoviruses in a cluster. These results support that this virus, which is tentatively named cherry latent virus 1, should be considered a new member in the genus Trichovirus.

中文翻译:

发现并感染甜樱桃的新型毛状病毒的分子特征。

通过高通量测序从无症状甜樱桃品种中鉴定出与杏假绿叶叶斑病毒(Trichovirus属,Betaflexiviridae属)具有最高序列相似性(73%)的重叠群。该新病毒的完整基因组序列为7460个核苷酸,不包括3'poly(A)尾巴。它的基因组组织与感染果树的多种毛滴虫病毒非常相似,具有三个开放阅读框,编码假定的复制酶,运动蛋白和外壳蛋白(CP)。该病毒与其他滴虫病毒在复制酶上的氨基酸序列同一性为60-73%,在CP处的氨基酸序列同一性为53-76%。系统发育分析将它和其他毛状病毒分组。这些结果支持该病毒暂定命名为樱桃潜伏病毒1,应被认为是滴虫病毒属的新成员。
更新日期:2020-02-17
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