A novel virus, Botryosphaeria dothidea fusarivirus 1 (BdFV1), was isolated from a fungal strain, SDAU11-86 of Botryosphaeria dothidea, and its complete genome sequence was determined. BdFV1 has a single-stranded positive-sense (+ssRNA) genome with 6,179 nucleotides, excluding the poly(A) tail. The genome of BdFV1 contains two putative open reading frames (ORFs). The first ORF encodes a large polyprotein of 1,544 amino acids (aa) with conserved RNA-dependent RNA polymerase and viral helicase domains. The second ORF encodes a putative 481-aa protein with unknown function. Sequence comparisons and phylogenetic analysis suggested that BdFV1 is a novel mycovirus belonging to the newly proposed family "Fusariviridae". This is the first report of a +ssRNA mycovirus in B. dothidea.

中文翻译：

---

新型fusarivirus感染植物病原性真菌Botryosphaeria dothidea的分子特征。

从真菌Botryosphaeria dothidea的SDAU11-86菌株中分离出一种新型病毒Botryosphaeria dothidea fusarivirus 1（BdFV1），并确定了其完整的基因组序列。BdFV1具有一个单链正义（+ ssRNA）基因组，具有6,179个核苷酸，不包括poly（A）尾巴。BdFV1的基因组包含两个假定的开放阅读框（ORF）。第一个ORF编码具有保守的依赖于RNA的RNA聚合酶和病毒解旋酶结构域的1,544个氨基酸（aa）的大型多蛋白。第二个ORF编码功能未知的推定481-aa蛋白。序列比较和系统发育分析表明，BdFV1是一种新的分枝杆菌病毒，属于新提出的家族“ Fusariviridae”。这是在双歧杆菌中+ ssRNA分支杆菌病毒的首次报道。