An investigation was carried out using sugarcane bagasse as the agricultural residue to study the optimization of xylanase production by solid-state fermentation. Maximum xylanase production (20.35 U/g substrate) was achieved by Bacillus substilis subsp. subtilis JJBS250 using 'one variable at a time approach' at pH 7.0, 40 °C after 48 h. After statistical optimization by response surface methodology (RSM) there was 4.82-fold improvement in xylanase production (98.16 U/g substrate). Further optimization of untreated and sodium carbonate pretreated sugarcane bagasse enzymatic hydrolysis was carried out using both bacterial (Bacillus substilis subsp. subtilis JJBS250) and fungal (Myceliophthora thermophila BJTLRMDU3) xylanases that showed high amount of reducing sugar liberation from untreated sugarcane bagasse (124.24 mg/g substrate) as compared to pretreated (76.23 mg/g substrate) biomass. Furthermore, biophysical characterization of untreated and sodium carbonate pretreated sugarcane bagasse using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM), revealed the structural changes in the pretreated biomass.

中文翻译：

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增强的细菌木聚糖酶生产及其在甘蔗渣糖化中的用途。

以甘蔗渣为农残进行了研究，以研究固态发酵法生产木聚糖酶的最优化。枯草芽孢杆菌亚种获得最大的木聚糖酶产量（20.35 U / g底物）。枯草芽孢杆菌JJBS250在48 h下在40°C的pH 7.0下使用“一次变量法”。通过响应面方法（RSM）进行统计优化后，木聚糖酶产量提高了4.82倍（98.16 U / g底物）。使用细菌木聚糖酶（Bacillus substilis subsp.subtilis JJBS250）和真菌木霉（Myceliophthora thermophila BJTLRMDU3）木聚糖酶对未经处理和碳酸钠预处理的甘蔗渣进行进一步的酶促水解，这表明从未经处理的甘蔗渣中大量还原糖释放（124。与预处理的生物质（76.23 mg / g底物）相比，底物为24 mg / g（底物）。此外，使用傅里叶变换红外光谱（FTIR），X射线衍射（XRD）和扫描电子显微镜（SEM）对未经处理的碳酸钠和经过碳酸钠预处理的甘蔗渣进行生物物理表征，揭示了经过预处理的生物质的结构变化。