Background: The essential oil of methyl eugenol rich Cymbopogon khasianus Hack. was evaluated and its bioactivities were compared with pure methyl eugenol. So far, methyl eugenol rich essential oil of lemongrass was not studied for any biological activities; hence, the present study was conducted.

Objective: This study examined the chemical composition of essential oil of methyl eugenol rich Cymbopogon khasianus Hack., and evaluated its antioxidant, anti-inflammatory, antimicrobial, and herbicidal properties and genotoxicity, which were compared with pure compound, methyl eugenol.

Material and Methods: Methyl eugenol rich variety of Cymbopogon khasianus Hack., with registration no. INGR18037 (c.v. Jor Lab L-9) was collected from experimental farm CSIR-NEIST, Jorhat, Assam (26.7378°N, 94.1570°E). The essential oil wasobtained by hydro-distillation using a Clevenger apparatus. The chemical composition of the essential oil was evaluated using GC/MS analysis and its antioxidant (DPPH assay, reducing power assay), anti-inflammatory (Egg albumin denaturation assay), and antimicrobial (Disc diffusion assay, MIC) properties, seed germination effect and genotoxicity (Allium cepa assay) were studied and compared with pure Methyl Eugenol compound (ME).

Results: Major components detected in the Essential Oil (EO) through Gas chromatography/mass spectroscopy analysis were methyl eugenol (73.17%) and β-myrcene (8.58%). A total of 35components were detected with a total identified area percentage of 98.34%. DPPH assay revealed considerable antioxidant activity of methyl eugenol rich lemongrass essential oil (IC50= 2.263 μg/mL), which is lower than standard ascorbic acid (IC50 2.58 μg/mL), and higher than standard Methyl Eugenol (ME) (IC50 2.253 μg/mL). Methyl eugenol rich lemongrass EO showed IC50 38.00 μg/mL, ME 36.44 μg/mL, and sodium diclofenac 22.76 μg/mL, in in-vitro anti-inflammatory test. Moderate antimicrobial activity towards the 8 tested microbes was shown by methyl eugenol rich lemongrass essential oil whose effectiveness against the microbes was less as compared to pure ME standard. Seed germination assay further revealed the herbicidal properties of methyl eugenol rich essential oil. Moreover, Allium cepa assay revealed moderate genotoxicity of the essential oil.

Conclusion: This paper compared the antioxidant, anti-inflammatory, antimicrobial, genotoxicity and herbicidal activities of methyl eugenol rich lemongrass with pure methyl eugenol. This methyl eugenol rich lemongrass variety can be used as an alternative of methyl eugenol pure compound. Hence, the essential oil of this variety has the potential of developing cost-effective, easily available antioxidative/ antimicrobial drugs but its use should be under the safety range of methyl eugenol and needs further clinical trials.

背景：富含甲基丁香酚的Cymbopogon khasianus Hack精油。进行了评估，并将其生物活性与纯甲基丁香酚进行了比较。到目前为止，尚未研究富含甲基丁香酚的柠檬草精油的任何生物活性。因此，进行了本研究。

目的：研究富含甲基丁香酚的Cymbopogon khasianus Hack。的精油的化学成分，并评估其抗氧化，抗炎，抗菌，除草性能和遗传毒性，并与纯化合物甲基丁香酚进行比较。

材料和方法：富含甲基丁香酚的Cymbopogon khasianus Hack。，注册号为。INGR18037（cv乔尔实验室L-9）是从阿萨姆邦乔哈特的实验农场CSIR-NEIST（26.7378°N，94.1570°E）收集的。使用Clevenger设备通过水蒸馏获得精油。使用GC / MS分析及其抗氧化剂（DPPH测定，还原力测定），抗炎（蛋清蛋白变性测定）和抗菌（碟片扩散测定，MIC）特性，种子发芽效果评估精油的化学组成。进行了遗传毒性和遗传毒性（洋葱头孢属测定）的研究，并与纯甲基丁香酚化合物（ME）进行了比较。

结果：通过气相色谱/质谱分析法在香精油（EO）中检测到的主要成分为甲基丁香酚（73.17％）和β-月桂烯（8.58％）。总共检测到35个成分，总鉴定面积百分比为98.34％。DPPH分析显示富含甲基丁香酚的柠檬草精油（IC50 = 2.263μg/ mL）具有相当大的抗氧化活性，低于标准抗坏血酸（IC50 2.58μg/ mL），但高于标准甲基丁香酚（ME）（IC50 2.253μg / mL）。在体外抗炎试验中，富含甲基丁香酚的柠檬草EO的IC50为38.00μg/ mL，ME为36.44μg/ mL，双氯芬酸钠为22.76μg/ mL。富含甲基丁香酚的柠檬草精油显示出对8种被测微生物的中等抗菌活性，与纯ME标准品相比，其对微生物的效力较低。种子发芽试验进一步揭示了富含甲基丁香酚的精油的除草特性。而且，洋葱头孢属测定法揭示了精油的中等遗传毒性。

结论：本文比较了富含甲基丁香酚的柠檬草与纯甲基丁香酚的抗氧化，抗炎，抗菌，遗传毒性和除草活性。这种富含甲基丁香酚的柠檬草品种可以用作甲基丁香酚纯化合物的替代品。因此，该品种的精油具有开发成本有效，易于获得的抗氧化/抗菌药物的潜力，但其使用应在甲基丁香酚的安全范围内，并且需要进一步的临床试验。