Porcine epidemic diarrhea virus (PEDV) is an emerging porcine coronavirus that causes a tremendous economic burden on the swine industry. The assessment of PEDV-neutralizing antibody levels provides a valuable tool to assess and predict herd immunity. We evaluated the performance of a PEDV imaging cytometry-based high-throughput neutralization test (HTNT) and compared the HTNT to a fluorescent focus neutralization (FFN) assay using serum samples from pigs of known PEDV infection status (n = 159). Estimates of diagnostic sensitivity and specificity for HTNT and FFN assays derived from receiver-operator characteristic (ROC) curve analyses showed that both PEDV FFN and HTNT provided excellent diagnostic performance. However, in the laboratory, imaging cytometry provided an objective and semi-automated approach that removed human subjectivity from the testing process and reduced the read-time of a 96-well plate to < 4 min. In addition, imaging cytometry facilitated the rapid collection and long-term storage of test images and data for further evaluation or client consultation. For PEDV and other pathogens, imaging cytometry could provide distinct advantages over classic virus neutralization or FFN assays for the detection and quantitation of neutralizing antibody.

中文翻译：

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使用高通量成像细胞术检测猪流行性腹泻病毒中和抗体。

猪流行性腹泻病毒（PEDV）是一种新兴的猪冠状病毒，对猪产业造成巨大的经济负担。PEDV中和抗体水平的评估为评估和预测牛群免疫性提供了有价值的工具。我们评估了基于PEDV成像，基于细胞计数的高通量中和测试（HTNT）的性能，并使用已知PEDV感染状态（n = 159）的猪的血清样本将HTNT与荧光焦点中和（FFN）分析进行了比较。从接收者-操作者特征（ROC）曲线分析得出的HTNT和FFN分析的诊断敏感性和特异性估计表明，PEDV FFN和HTNT均提供了出色的诊断性能。但是在实验室里 成像细胞计数法提供了一种客观且半自动化的方法，该方法从测试过程中消除了人的主观性，并将96孔板的读取时间缩短至<4分钟。此外，成像细胞计数有助于快速收集和长期保存测试图像和数据，以进行进一步评估或咨询客户。对于PEDV和其他病原体，与传统的病毒中和法或FFN测定法相比，成像细胞计数法可提供独特的优势来检测和定量中和抗体。