We investigated the effects of artemisinin on doxorubicin (Dox) induced heart and liver pathology in rats. We divided 49 male rats into seven groups: group 1 was the untreated control. Dox was administered intraperitoneally to groups 2, 3 and 4 on day 1. Artemisinin was administered by gavage to groups 3 and 6 at a dose of 7 mg/kg, and to groups 4 and 7 at a dose of 35 mg/kg for 14 days. Group 5 was given only 0.9% NaCl orally for 14 days. At the end of the study, heart and liver samples were collected for histopathology and immunohistochemistry. Hyperemia and slight hemorrhages were observed in both livers and hearts of rats treated with Dox only. Significant increases in caspase-3, TNF-α, iNOS and NF-κB expression were observed in the myocardial cells and hepatocytes of group 2. Significant reductions in caspase-3, TNF-α, iNOS and NF-κB expression were observed in groups 3 and 4 following artemisinin treatment compared to group 2. Artemisinin may exert protective effects against Dox induced cardiotoxicity and hepatotoxicity in rats.

中文翻译：

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青蒿素减弱阿霉素诱导的大鼠心脏毒性和肝毒性。

我们研究了青蒿素对阿霉素 (Dox) 诱导的大鼠心脏和肝脏病理学的影响。我们将 49 只雄性大鼠分为七组：第 1 组是未处理的对照组。第 1 天对第 2、3 和 4 组腹膜内给予 Dox。第 3 和第 6 组以 7 mg/kg 的剂量通过管饲法给予青蒿素，第 4 和第 7 组以 35 mg/kg 的剂量给予青蒿素，持续 14天。第 5 组仅口服 0.9% NaCl，持续 14 天。在研究结束时，收集心脏和肝脏样本用于组织病理学和免疫组织化学。在仅用 Dox 治疗的大鼠的肝脏和心脏中均观察到充血和轻微出血。在第 2 组的心肌细胞和肝细胞中观察到 caspase-3、TNF-α、iNOS 和 NF-κB 表达显着增加。 caspase-3、TNF-α、