当前位置：
X-MOL 学术
›
Mol. Reprod. Dev.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Ratio of the zygote cytoplasm to the paternal genome affects the reprogramming and developmental efficiency of androgenetic embryos.
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-02-16 , DOI: 10.1002/mrd.23327 Chen Liao 1 , Xinghui Shen 1 , Yuwei Zhang 2 , Lei Lei 1
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-02-16 , DOI: 10.1002/mrd.23327 Chen Liao 1 , Xinghui Shen 1 , Yuwei Zhang 2 , Lei Lei 1
Affiliation
|
Uniparental embryos have uniparental genomes and are very useful models for studying the specific gene expression of parents or for exploring the biological significance of genomic imprinting in mammals. However, the early developmental efficiency of androgenetic embryos is significantly lower than that of parthenogenetic embryos. In addition, oocytes are able to reprogram sperm nuclei after fertilization to guarantee embryonic development by maternally derived reprogramming factors, which accumulate during oogenesis. However, the importance of maternal material in the efficiency of reprogramming the pronucleus of androgenetic embryos is not known. In this study, androgenetic embryos were constructed artificially by pronucleus transfer (PT) or double sperm injection (DS). Compared with DS embryos, PT embryos that were derived from two zygotes contained more maternal material, like 10-11 translocation methylcytosine deoxygenase 3 (Tet3) and histone variant 3.3 (H3.3). Our experiments confirmed the better developmental potential of PT embryos, which had higher blastocyst rates, a stronger expression of pluripotent genes, a lower expression of apoptotic genes, and superior blastocyst quality. Our findings indicate that the aggregation of more maternal materials in the paternal pronucleus facilitate the reprogramming of the paternal genome, improving embryonic development in PT androgenesis.
中文翻译：
合子细胞质与父本基因组的比率影响雄激素胚的重编程和发育效率。
单亲胚胎具有单亲基因组，对于研究父母的特定基因表达或探索哺乳动物基因组印迹的生物学意义而言，是非常有用的模型。但是，雄性胚的早期发育效率明显低于孤雌生殖的胚。此外，卵母细胞受精后能够重编程精子核，从而通过母体衍生的重编程因子来保证胚胎发育，该因子在卵子发生过程中积累。然而，母体材料在重编程雄激素胚胎前核效率中的重要性尚不清楚。在这项研究中，通过原核移植（PT）或双精子注射（DS）人工构建雄激素胚。与DS胚胎相比，来自两个受精卵的PT胚胎含有更多的母体物质，例如10-11易位的甲基胞嘧啶脱氧酶3（Tet3）和组蛋白变体3.3（H3.3）。我们的实验证实了PT胚胎具有更高的发育潜力，其胚泡率更高，多能基因表达更强，凋亡基因表达更低，胚泡质量更高。我们的发现表明，父本核中更多母本物质的聚集促进了父本基因组的重新编程，改善了PT雄激素形成中的胚胎发育。多能基因的表达更强，凋亡基因的表达更低，胚泡质量更高。我们的发现表明，父本核中更多母本物质的聚集促进了父本基因组的重编程，改善了PT雄激素形成中的胚胎发育。多能基因的表达更强，凋亡基因的表达更低，胚泡质量更高。我们的发现表明，父本核中更多母本物质的聚集促进了父本基因组的重编程，改善了PT雄激素形成中的胚胎发育。
更新日期：2020-02-16
中文翻译：
合子细胞质与父本基因组的比率影响雄激素胚的重编程和发育效率。
单亲胚胎具有单亲基因组，对于研究父母的特定基因表达或探索哺乳动物基因组印迹的生物学意义而言，是非常有用的模型。但是，雄性胚的早期发育效率明显低于孤雌生殖的胚。此外，卵母细胞受精后能够重编程精子核，从而通过母体衍生的重编程因子来保证胚胎发育，该因子在卵子发生过程中积累。然而，母体材料在重编程雄激素胚胎前核效率中的重要性尚不清楚。在这项研究中，通过原核移植（PT）或双精子注射（DS）人工构建雄激素胚。与DS胚胎相比，来自两个受精卵的PT胚胎含有更多的母体物质，例如10-11易位的甲基胞嘧啶脱氧酶3（Tet3）和组蛋白变体3.3（H3.3）。我们的实验证实了PT胚胎具有更高的发育潜力，其胚泡率更高，多能基因表达更强，凋亡基因表达更低，胚泡质量更高。我们的发现表明，父本核中更多母本物质的聚集促进了父本基因组的重新编程，改善了PT雄激素形成中的胚胎发育。多能基因的表达更强，凋亡基因的表达更低，胚泡质量更高。我们的发现表明，父本核中更多母本物质的聚集促进了父本基因组的重编程，改善了PT雄激素形成中的胚胎发育。多能基因的表达更强，凋亡基因的表达更低，胚泡质量更高。我们的发现表明，父本核中更多母本物质的聚集促进了父本基因组的重编程，改善了PT雄激素形成中的胚胎发育。
京公网安备 11010802027423号